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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

1-ETHYL-3-METHYL IMIDAZOLIUM ETHYLSULFATE induced no visible effects in carp at or below 100 mg/L (NOEC). The 96h-LC50 was > 100 mg/L based on analytically confirmed nominal concentrations.

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Additional information

The study procedure described in this report was based on the ISO International Standard 7346-1: Static method, 1996. In addition, the procedures were designed to meet the test methods and validity criteria of the EEC directive 92/69, Part C.l ,1992, the OECD guideline No. 203, 1992 and the OECD series on testing and assessment number 23, 2000. The batch of 1-ETHYL-3-METHYL IMIDAZOLIUM ETHYLSULFATE tested was a light yellow viscous liquid with a purity of 99.0% and completely soluble in test medium at the concentrations tested. Preparation of the test solution started with a stock solution of 100 mg/L applying 16 minutes of magnetic stirring to accelerate the dissolving of the test substance in the test medium. The final test solution was clear and colourless. A limit test was performed, exposing seven fish per concentration to a blank-control and a nominal concentration of l00 mg/L. The total test period was 96 hours and samples for analytical confirmation of actual test concentrations were taken from the blank-control and the limit concentration at the start, after 24 hours of exposure and at the end of the test period. Analysis of the samples taken at the start of the limit test showed that the mean actual test concentration was in agreement with nominal (104%) and remained stable during the 96-hour test period (i.e. 1 02% of initial). Based on these results, the NOEC and the LC50 were based on nominal concentrations. The study met the acceptability criteria prescribed by the protocol and was considered valid. EMIM ES induced no visible effects in carp at or below 100 mg/L (NOEC). The 96h-LC50 was> 100 mg/L based on analytically confirmed nominal concentrations.

This is further supported by an acute toxicity study in zebra fish demonstrating no toxicity up to a concentration of 1-ETHYL-3-METHYL IMIDAZOLIUM ETHYLSULFATE of 150 mg/L.