Registration Dossier

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

Assessment of Benzenepropanoic acid, 3,5-bis(1,1-dimethylethyl)-4-hydroxy-,C7-9-branched alkyl esters (main constituent, parent):

Test organism

Test duration

Effect parameter

Effect value (mg/L)

Nominal/
measured

OECD guideline

Vehicle

Year of the study

 

Remarks

 

Brachydanio rerio

96h

LC50

>100

nominal

203

yes

1989

  

No effects in the range of solubility

Oncorhynchus mykiss

14d

NOEC

>=100

nominal

204

no

2000

  

No effects in the range of solubility

Brachydanio rerio

35d

NOEC

>=0.001

nominal

210

yes

2007

  

No effects in the range of solubility

Daphnia magna

48h

LC50

>100

nominal

202

no

2002

  

No effects in the range of solubility

Daphnia magna

21d

NOEC

1:3.2 dilution

loading rate 100mg/L

211

no

2000

  

Effects at the highest test concentration

Daphnia magna

21d

NOEC

>= 1mg/L

loading rate 1mg/L

211

no

2012

  

No effects in the range of solubility

Scenedesmus subspicatus

72h

LC50

NOEC

>3

>= 3

nominal

201

yes

1995

 

No effects in the range of solubility

 
 Activated sludge  3h  EC50  > 100mg/L  nominal  209  no  1989  

No toxic effects of Benzenepropanoic acid, 3,5-bis(1,1-dimethylethyl)-4-hydroxy-,C7-9-branched alkyl esters were observed in the range of solubility. The inhibition of the degradation activity of activated sludge is not anticipated when introduced in appropriate low concentrations. The substance is with high probability acutely and chronically not harmful to aquatic organisms.

Supporting assessment of major degradation product Metilox acid (CAS 20170-32-5)

 Test organism  Test duration Effect parameter Effect value Nominal/measured  OECD guideline Vehicle  Year of the study   Remarks 
 Brachydanio rerio  96h  LC50  130 mg/L  nominal  203  yes  1989   A slight deposit was observed at conc. 180-320 mg/l (nominal) from the start of the test.
 Daphnia magna  48h  LC50  > 121 mg/L  measured  202  no  2011  
 Pseudokirchnerella subcapitata  72h  ErC50 ErC10  >100mg/L 95mg/L  nominal  201  no  2009  
  Activated sludge  3h  EC50  > 100mg/L  nominal  209  no  1988  

Acute toxicity test results are available for fish, Daphnia and algae. In an activated sludge respiration inhibition test, no adverse effects were observed. Slight toxic effects were observed in tests with fish and algae. However, these effects were caused by very high concentrations and are above the criteria for classification according to CLP.

Supporting assessment of the impurity Metilox (CAS 6386-38-5)

In a 96-h acute toxicity study to zebra-fish (Brachydanio rerio) under static conditions, the 96-h LC50 was 5.6 mg/L.  The LC0, based on mortality was 3.2 mg/L. 

In a 24-h acute toxicity to Daphnia magna under static conditions. The 24 -h EC50 was > 100 mg/L, the 24 -h EC0 based on immobilization was 5.8 mg/L.   

In a long-term toxicity to aquatic invertebrates test with Daphnia under semi-static conditions, the NOEC based on reproduction was 0.123 mg/L based on measured concentration and 0.16 mg/L based on nominal concentraions.

In a 72 h acute toxicity study to Scenedesmus subspicatus the NOEbC and EbC50 values based on cell number were 2.3 and 0.5 mg/L, respectively. 

In a 3 h acute toxicity study to sewage sludge microorganisms the IC50 value based on respiration rate was bigger than 100 mg/L after a 3 h contact time.

 

Supporting assessment of impurity 1 octanol (CAS 111 -87 -5)

Acute toxicity tests results are available for freshwater fish (Pimephales promelas), invertebrates (Daphnia magna) and algae (Pseudokirchnerella subcapitata). LC50 or EC50 values for the three organisms were 13.3, 20 and 14 mg/L respectively.

In waste-water treatment plant micro-organisms, octan-1-ol does not cause inhibition at the limit of water solubility. In view of the ready biodegradability, further information is not required in REACH.

A NOEC value of 1.0 mg/L (21 day reproduction) has been determined for the effects of octan-1-ol on reproduction and survival of the freshwater test organism Daphnia magna.

(ECHA disseminated data, August 04, 2015)