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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
June 2007
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
Samples of the test media for determination of the actual test substance concentrations were taken immediately before introduction of the Daphnia and after 48 hours. Actual concentrations of the test substance in the test media were determined in the surplus of the test media at the beginning of the test and in the pooled test media of the replicates at the end of the test.
One sample each was taken and then analysed in duplicate by HPLC. The samples were
analysed at the day of sampling.
Details on test solutions:
Identity and concentration of auxiliary solvent for dispersal: A suspension of 16 mg/L nominal concentration (7.0 mg/L geometric mean) of the test substance was prepared in water by stirring for approximately 48 hours in the dark and 15 minutes of ultrasonication followed by filteration (0.2 µm) prior to the test start.
Test organisms (species):
Daphnia magna
Details on test organisms:
Neonates of Daphnia magna (Crustacea, Cladocera), less than 24 hours old, were obtained by hatching ephippia, supplied in the microbiotest "Daphtoxkit F TM " (Daphnia magna;
MicroBioTests Inc., Industriezone "De Prijkels", Venecoweg 19 , 9810 Nazareth, Belgium).
The ephippia were hatched in the laboratory in a petri dish containing reconstituted water in a temperature controlled room at approx. 20-22 °C under continuous illumination of
approximately 6 000 Lux.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Remarks on exposure duration:
Animal observation 24 and 48 hours after the start of the test
Hardness:
250 mg CaCO3/L
Test temperature:
20°C
pH:
7.5 and 7.2
Dissolved oxygen:
Dissolved oxygen concentration: at least 8.5 mg/L.
Nominal and measured concentrations:
As the test substance concentration declined by 22.3 % from 0 to 48 h, the calculation of the effective concentrations was based on the geometric means of the actual test substance concentrations determined at the start and at the end of the incubation.

Actual concentration at: 0 h (mg/L) 48 h (mg/L)
7.95 6.17
Reference substance (positive control):
no
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 7 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 7 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 7 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
ca. 7 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility

No daphnia were immobilised, neither in the test substance group nor in the control group.

Two daphnia were trapped at the water surface in the test substance group at 24 h and one

at 48 h. No control daphnia were trapped at the water surface.

Validity criteria fulfilled:
yes
Executive summary:

Effective concentrations based on the actual test substance concentration.

The actual test substance concentration is based on the geometric mean of the actual test substance concentration measured at the start and at the end of the test:

 Parameter  

Actual test substance concentration (mg/L)

EC50,24h

  >7.0

  EC50,48h

  >7.0

  EC100,48h

  >7.0

   EC0,48h

≥7.0 

 NOEC48h

 7.0

Description of key information

Effective concentrations based on the actual test substance concentration.

The actual test substance concentration is based on the geometric mean of the actual test substance concentration measured at the start and at the end of the test:

 Parameter  

Actual test substance concentration (mg/L)

EC50,24h

  >7.0

  EC50,48h

  >7.0

  EC100,48h

  >7.0

   EC0,48h

≥7.0 

 NOEC48h

 7.0

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
7 mg/L

Additional information