2-phthalimidoethyl N-[4-(2-cyano-4-nitrophenylazo)phenyl]-N-methyl-β-alaninate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From October 18, 1996, to March 28, 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

1992

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Red RA 10463

IUPAC Name:

Red RA 10463

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Start and end of study period

Test solutions

Vehicle:

yes

Details on test solutions:

Since the substance is practically insoluble in water (<9 µg/l), 0.1 ml/l acetone was used as a pre-solvent in the stock solution. Nevertheless, the final test solutions were pink with undissolved test item particles floating at the surface.

Test organisms

Test organisms (species):

Cyprinus carpio

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Test temperature:

20.5 - 21.0 °C

pH:

7.2 - 8.2

Dissolved oxygen:

6.6 - 9.8 mg O2/l

Nominal and measured concentrations:

- Nominal concentration: 1.0 mg/l limit test
- Measured concentration: 1.07 mg/l at the start of the test; 0.26 mg/l at the end

Reference substance (positive control):

yes

Remarks:

pentachlorophenol, 0.1 - 1.0 mg/l

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

not determinable

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality occurred in the treatment, control or solvent control groups. Concentration levels acutely toxic for this species of fish could not be reached, owing to the very low solubility of the test item in water (< 9 µg/l at 20.0 ± 0.5 °C). Therefore, the 96 hour LC50 value was far beyond the maximum solubility in water.

Results with reference substance (positive control):

LC50 (96h) = 0.42 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

LC50 (96h, Cyprinus carpius) > maximum solubility limit
No mortality at the highest tested concentration of 1.0 mg/l (nominal).