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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report date:
2009

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
471-140-1
EC Name:
-
Cas Number:
121240-56-0
Molecular formula:
C16H37F2P
IUPAC Name:
hydrogen tetrabutylphosphanium difluoride

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Concentration measured at 0 and 72 hours

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
Non-axenic strain from "The Collection of algal Cultures" of the Institute of Plant Physiology at the University of Goettingen (Germany)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
23 +/- 2 °C
pH:
pH range: 8.2/8.7 (at test start/after 72 h)
Nominal and measured concentrations:
0.088, 0.19, 0.43, 0.94, 2.1, 4.5 and 10 mg/L (nominal) plus 1 control

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.088 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
1.1 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
5.2 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.088 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
0.13 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
1.1 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
biomass
Details on results:
NOEC and LOEC were determined according to Williams Multiple Sequential t-Test

Any other information on results incl. tables

The results are expressed in terms of nominal concentrations. Recovery rates ranged from 94.3-115.3% of nominal values at 0 hours, and from 95.1-117.8% of nominal values at 72 hours, respectively.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

The toxicity of Tetrabutylphosphoniumhydrogendifluoride to algae (Desmodesmus subspicatus) was assessed performing a study according to OECD TG 201 (Currenta 2009). The following results were determined: Inhibition of the algal population as reduction in growth rate was 72 h-ErC50 =  5.2 mg/l. The No Observed Effect Concentration (NOEC) was 0.088 mg/l. The results are expressed in terms of nominal concentrations.