Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 1994-09-14 to 1994-11-16
Reliability:
1 (reliable without restriction)
Cross-reference
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report Date:
1994

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
Directive 92/69/EEC
Deviations:
no
Principles of method if other than guideline:
NA
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid: viscous
Details on test material:
NA
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
NA

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Frequency: at t=0h and t=48h
Volume: 10 mL from the approximate centre of the test vessel

Test solutions

Vehicle:
yes
Details on test solutions:
The test solution, concentration 100 mg/L, was prepared by adding quantitatively 24.8 mg test substance to 250 mL ISO medium. The final test solution was clear without precipitation.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
For the test selection of young daphnia with an age of < 24 hours.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
NA

Test conditions

Hardness:
250 mg CaCO3/L
Test temperature:
The temperature measured in blank control was 21 °C.
pH:
8.0 +/- 0.2
Dissolved oxygen:
between 8.5 and 9.1 mg O2/L
Salinity:
NA
Nominal and measured concentrations:
Analysis of the sample taken at the start (t=0) of the final test revealed an actual concentration of 113 mg/L (113%) at the nominal concentration of 100 mg/L. During the exposure period the actual concentration remained above 80 % of the initial.
Details on test conditions:
NA
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
NA
Results with reference substance (positive control):
Concentrations: 0.00, 0.10, 0.18, 0.32, 0.56 and 1.0 mg/L in ISO-medium (without test substance)
The EC50 after 24 and 48 hours was between 1.0 and 1.8 mg/L.
Reported statistics and error estimates:
NA

Any other information on results incl. tables

no remarks

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
EC50 > 100 mg/L
Executive summary:

The 48h-EC50 of the test item for immobilization of daphnia was greater than 100 mg/L, the maximum concentration to be tested.