Butanamide, N-(3-ethoxypropyl)-2,4-dihydroxy-3,3-dimethyl-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1994-08-31 to 1994-09-29

Reliability:

1 (reliable without restriction)

Cross-reference

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Deviations:

no

Principles of method if other than guideline:

NA

GLP compliance:

yes

Test material

Test material information

Constituent 1

Test material form:

liquid: viscous

Details on test material:

NA

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
NA

Study design

Oxygen conditions:

anaerobic

Inoculum or test system:

activated sludge, industrial, non-adapted

Details on inoculum:

The source of the test organisms was activated sludge freshly obtained from a multicipal sewage treatement plant. The sludge was kept under conditions aeration until further treatment. The concentration of suspended solids was 3.8 g/L in the concentrated sludge. Before use, the sludge was allowed to settle for at least 30 minutes and the liquid decanted for use as inoculum at the amount of 10 mL/L of mineral medium.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

26.8 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

NA

Reference substance

Reference substance:

other: sodium acetate

Results and discussion

Preliminary study:

NA

Test performance:

NA

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

> 10 - < 12

Sampling time:

28 d

Details on results:

The relative degradation values calculated from the measurements performed during the test period revealed no significant (> 10 %) degradation of the test item in bottle A, and very slight degradation (12%) in bottle B.

BOD5 / COD results

Results with reference substance:

The control substance was degraded 82 % in 14 days and the complete degradation was reached at the end of the test period.

Any other information on results incl. tables

No remarks

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test substance was not readily biodegradable.

Executive summary:

Under the conditions of activated sludge respiration test the test item was found to be not toxic to waste-water bacteria at 100 mg per litre. Therefore, the test item was assumed to be not inhibitory at the concentrations used in the modified Sturm test presently performed. The test item was not readily biodegradable under the conditions of the modified Sturm test presently performed.