Registration Dossier

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
25 June 1998
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1998
Report Date:
1998

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): (R)-(+)-1-Phenylethylamin
- Substance type: active substance
- Physical state: liquid
- Analytical purity: 99.6 area-% (analytical report: ZAX 98L00289)
- Lot/batch No.: 68-0655 (date of production: 09 Feb. 1997)
- Stability under test conditions: instable against acids and alkali
- Storage condition of test material: room temperature
- Other:

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The substance was added directly to the test vessels.

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Laboratory culture: yes, municipal activated sludge taken from a laboratory wastewater treatment plant fed with municipal sewage.
- Preparation of inoculum for exposure: The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated
for 24 hours. 50 mL were added to a total volume of 250 mL to obtain a concentration of 1 g/L dry substance in the test.

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
30 min

Test conditions

Test temperature:
20±2 °C
Dissolved oxygen:
Oxygen concentration during aeration: > 2.5 mg/L
Oxygen concentration immediately before measurement of oxygen consumption: > 6.5 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer-vessel
- Material, size, headspace, fill volume: glass, nominal volume: 250 mL, fill volume 250 mL
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic medium: 8 mL/vessel 100-fold concentrated OECD medium

OTHER TEST CONDITIONS
- Adjustment of pH: yes
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
30 min
Dose descriptor:
other: EC20
Effect conc.:
ca. 550 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
- Results with reference substance valid?: yes
- Relevant effect levels: 30-min EC20: ca. 1.8 mg/L; 30-min EC50: ca. 6.7 mg/L; 30-min EC80: ca. 60 mg/L

Any other information on results incl. tables

Respiration data:

Treatment

BC1

BC2

BC3

BC

(mean value)

TS1

TS2

TS3

RS1

RS2

RS3

Concentration of test substance

(mg/L)

-

-

-

-

1000

500

250

1

10

100

O2 consumption rate

(mg O2/L*h)

30

28

28

29

18

24

26

27

12

4

Specific O2 consumption rate

(mg O2/g*h)

30

28

28

29

18

24

26

27

12

4

Change in oxygen consumption

rate compared to blank control

(%)

-

-

-

-

-38

-17

-10

-7

-59

-86

pH value*

6.4

6.5

6.6

 

9.8

9.6

8.9

6.5

6.6

6.5

* The pH value has been measured and adjusted to pH 7.0 to 8.0 before the addition of activated sludge.

Applicant's summary and conclusion