Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
01 June 1984 to 05 June 1984
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: guideline study with acceptable restrictions (e.g. test item concentrations not analytically verified)

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1984
Report Date:
1984
Reference Type:
secondary source
Title:
Unnamed
Year:
1992

Materials and methods

Principles of method if other than guideline:
- ASTM, standard practice for conducting acute toxicity tests with fishes, macroinvertebrates, and amphibians, E 729-80, 25 pp, 1980
- US EPA, methods for acute toxicity tests with fish, macroinvertebrates, and amphibians. Ecological Research Series (EPA-660/33-75-009), 61 pp, 1975
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): PEA [L-(-)-alpha phenyl ethylamine]
- Physical state: colourless liquid

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution (18,000 mg/L; test substance in deionised water)

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
TEST ORGANISM
- Common name: Fathead Minnow
- Source: SBI Wareham, Massachusetts Laboratory, USA
- Age at study initiation (mean and range, SD): 5 to 6 weeks
- Length at study initiation (length definition, mean, range and SD): 11-19 mm (13 ± 2 mm)
- Weight at study initiation (mean and range, SD): 0.01 to 0.06 g (0.02 ± 0.02 g)
- Feeding during test: no

ACCLIMATION
- Acclimation period: 14 d
- Acclimation conditions (same as test or not): similar, temperature range slightly lower (19 to 21 °C)
- Type and amount of food: live brine shrimp nauplii and/or commercial flake food
- Feeding frequency: daily
- Health during acclimation (any mortality observed): 0% in the 48-h period before test start

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
23 mg/L as CaCO3
Test temperature:
21-23 °C
pH:
7.0 to 9.4
Dissolved oxygen:
5.6 to 9.6 mg/L
Nominal and measured concentrations:
nominal: 7.8, 13, 22, 36, 68, 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 3.8-L glass test container, covered, fill volume: 3 L, water depth: 17 cm
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 3.3 fish/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 14 h light/10 h dark
- Light intensity: fluorescent lighting, 450 foot candles

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- mortality after 24, 48, 72 and 96 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: < 2
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study
- Results used to determine the conditions for the definitive study: yes

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
17 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% CL: 13-22 mg/L
Reported statistics and error estimates:
LC50 determined using the following method: non-linear interpolation with 95% confidence limits estimated by binomial probability.

Any other information on results incl. tables

Mortality:

Test item conc.

Cumulative mortality (%)

(nominal; mg/L)

24 h

48 h

72 h

96 h

0

0

0

0

0

7.8

0

0

0

0

13

0

0

0

0

22

100

100

100

100

36

100

100

100

100

60

100

100

100

100

100

100

100

100

100

pH and dissolved oxygen:

Test item conc.

pH (replicate A)

Dissolved oxygen (mg/L)

(nominal; mg/L)

initial

final

 

0

7.3

7.0

6.4 to 8.8

7.8

8.5

7.0

6.7 to 9.6

13

8.7

7.0

6.8 to 8.9

22

not measured

7.9

5.6

36

9.1

8.4

6.3 to 8.8

60

not measured

8.8

7.8 to 7.9

100

9.4

8.9

8.0 to 8.9

Other physicochemical parameters:

- specific conductivity: 150 to 165 µmhos/cm

- total hardness: 28 to 40 mg/L as CaCO3

- total alkalinity: 64 to 178 mg/L (increasing values with increasing test item concentration)

Applicant's summary and conclusion