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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

Observed effects against algae were 72h-EC10 = 0.207 mg/L and 72h-EC50 = 0.998 mg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:
0.998 mg/L
EC10 or NOEC for freshwater algae:
0.207 mg/L

Additional information

In order to test toxicity to algae of the substance, a study was conducted in accordance with Commission Regulation (EC) No 761/2009 amending Regulation No 440/2008, Method C.3 ‘Freshwater Alga and Cyanobacteria, Growth inhibition test’ (2009) which is equivalent to OECD Guideline for Testing of Chemicals No. 201 (2006).

Desmodesmus subspicatus was exposed to the test solution of 7 concentration of the test substance (Nominal: 0.18, 0.39, 0.85, 1.9, 4.1, 9.1 and 20 mg/L) and blank control solution for a period of 72 h under static conditions. The cell densities were measured at 24 hour intervals. Inhibition of the algal population was measured as reduction in growth rate (index r), relative to control cultures grown under identical conditions. The results are expressed in terms of geometric mean measured concentrations. Effective concentrations ranged from 95.7 to 97.5 % of nominal values at 0 hours, and from 3.5 to 61.1 % of nominal values at 72 hours. This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute algae study.