Registration Dossier

Administrative data

Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2008
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report date:
2008

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 105 (Water Solubility)
Deviations:
not specified
GLP compliance:
no
Type of method:
flask method

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzamide, 3-amino-N-[4-(aminocarbonyl)phenyl]-4-methoxy-, hydrochloride
EC Number:
618-990-3
Cas Number:
939961-06-5
Molecular formula:
C15H15N3O3xClH
IUPAC Name:
Benzamide, 3-amino-N-[4-(aminocarbonyl)phenyl]-4-methoxy-, hydrochloride
Details on test material:
purity: 95.4 %
contains about 4.6% water

Results and discussion

Water solubility
Water solubility:
969 mg/L
Temp.:
20 °C
pH:
ca. 2.1

Any other information on results incl. tables

Preliminary Test: Water solubility below 1 g/L

Solubility test:

Three flasks containing approx. 4 g/l test substance in water were stirred for 5 h at 30 °C. Subsequently the flasks were stirred at 20 °C for 1 day, 2 days and 3 days respectively. After each day the content of one flask was filtered through a 0,45 μm membrane filter. The content of the test substance in the filtrate was determined by HPLC.

Flask No. Equilibration time pH of aqueous Test substance conc.

at 20 °C [h] solution [mg/l]

1                     24                                   2.1                            970

2                     48                                   2.1                            968

3                     72                                   2.1                            970

mean               --                                   --                               969

HPLC

Column : Synergi Polar RP, 250 mm x 4,6 mm ID, 4 μm Eluent A : acetonitrile/water with 0,05mol/l tetrabutyl ammonium hydrogensulfate = 5/95 Eluent B : acetonitrile/ water = 95/5

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): moderately soluble (100-1000 mg/L)
The water solubility of the substance is 969 mg/L at 20°C.
Executive summary:

The water solubility of the substance was determined according to OECD guideline 105 with the flask method. It was found to be 969 mg/L at 20 °C.