Zirconium di(acetate) oxide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Zirconium di(acetate) oxide

EC Number:

225-924-3

EC Name:

Zirconium di(acetate) oxide

Cas Number:

5153-24-2

Molecular formula:

C4 H6 O5 Zr

IUPAC Name:

zirconium di(acetate) oxide

Details on test material:

- Name of test material (as cited in study report): Zirkonium-Acetat I.LSG.B.ZRO2 P.EP
- Physical state: Liquid colourless; turbid
- Analytical purity: Zirconium di(acetate)oxide: 36,6 TI
- Impurities (identity and concentrations): Water: 63.4 TI
- Batch No.: P 51

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was stirred in M4 medium for about 20 hours at 20 ± 2 °C. Undissolved test substance was removed by centrifugation (approx. 20 min. at about 17700 G).
- Differential loading: yes

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: supplied by the Institut National de Recherche Chimique Appliqueé, France, in 1978: cultured and bred continuously in the Laboratory Experimental Toxicology and Ecology of BASF SE in Ludwigshafen, Germany
- Age at study initiation: 2-24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2,42 mmol/L

Test temperature:

19.1 - 19.6 °C

pH:

7,5 - 8,2

Dissolved oxygen:

8.4 -9.6 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 12.5, 25, 50, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, nominal volume, test volume: glass tubes with flat bottom, 20 ml, 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels for the measurement of pH and O2 (replicates): 1
- Biomass loading rate: 0.5 animals/ml
- Feeding: none

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water (M4 medium)
- Alkalinity: 0.84 mmol
- Ca/mg ratio: 4:1
- Conductivity: 603 µS/cm
- Intervals of water quality measurement: temperature continuously during the whole test period in a separate vessel close the test vessels; pH and oxygen content after 0h in an additional replicate without daphnids and after 48h in replicate 1 of each tested
concentration.

OTHER TEST CONDITIONS
- Photoperiod: day-night rhythm = 16 :8 h
- Light intensity: ca. 1-8 µE/(m²*s) at a wave length of 400 -750 nm

EFFECT PARAMETERS MEASURED: mobility after 0, 24, 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Details on results:

EC50 (24h) = 1.21 mg/L (last control experiment: 20 Sep 2004)