Tetrahydro-4-methylene-2-(2-methyl-1-propenyl)-2H-pyran

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Tetrahydro-4-methylene-2-(2-methyl-1-propenyl)-2H-pyran

EC Number:

262-475-2

EC Name:

Tetrahydro-4-methylene-2-(2-methyl-1-propenyl)-2H-pyran

Cas Number:

60857-05-8

Molecular formula:

C10H16O

IUPAC Name:

4-methylidene-2-(2-methylprop-1-en-1-yl)oxane

Details on test material:

- Chemical name of test material:tetrahydro-4-methylene-2-(2-methyl-1-propenyl)-2H-pyran
- Lot/batch No.: LJ 1-28/29
- Analytical purity: 99.2 area-%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solutions were stirred with a magnetic stirrer for about 10 min at approximately 20 ± 2 °C. Each test concentration was prepared separately.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): < 24 hours

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48

Test conditions

Hardness:

2.48 mmol/l

Test temperature:

20.3 – 20.7 °C

pH:

8.0 - 8.1

Dissolved oxygen:

8.3 - 8.8 mg/l

Nominal and measured concentrations:

Nominal test substance concentrations:
0, 5.0, 10, 22, 50 and 100 mg/l

Mean measured concentrations:
4.94, 10.2, 20.5, 46.9, 96.8 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass tubes (23 ml)
- Fill volume: 23 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.22 (animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium, prepared according to OECD guideline 202
- Ca/mg ratio: 4:1
- Conductivity: 606 μS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: 20 – 850 Lux at a wave length of 400 - 750 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 hours

TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study: EC50 after 48 h: 20-50 mg/l

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

33.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

For the statistical calculation / evaluation of the EC50 the probit method according to Finney with SAS (Version 8.2) software was used.