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EC number: 421-950-6
CAS number: 187674-70-0
3 h EC50 > 100 mg/L in activated sludge, OECD 209, L'Haridon 1999
The potential of the test material to cause toxicity to microorganisms
was determined in the key activated sludge respiration inhibition test
perofrmed by L'Haridon (1999) using activated sludge from a
predominantly domestic sewage. The study was conducted under GLP
conditions and in accordance with the standardised guideline OECD 209. Based
on the principles for assessing data quality defined by Klimisch et al.
(1997), the study was assigned a reliability score of 1.
The activated sludge was exposed to the test material at concentrations
of 0, 1, 3.16, 10, 31.6 and 100 mg/L for 3 hours under static conditions
during which time the culture was aerated. After 3 hours of
aeration/contact time, the contents of the test flasks were poured into
the measuring apparatus and the oxygen concentration was determined for
a period of up to 10 minutes. The activated sludge was also exposed to a
positive control, the reference material 3,5-dichlorophenol at
concentrations of 4, 12 and 36 mg/L.
There was no inhibition of the inoculum respiration rate measured for
each test concentration in comparison to the blank control. The
reference substance displayed sufficient inhibition to confirm the
viability of the inoculum.
Under the conditions of the test, the 3 h EC50 was determined to be >
100 mg/L for activated sludge. Therefore the test material should be
considered as non-toxic for the micro-organisms in a water treatment
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