Cyclododeca-1,5,9-triene

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Original reference not yet available

Data source

Reference

Reference Type:

publication

Title:

Robust summary for 1,5,9-cyclododecatriene (revised).

Author:

DuPont Safety, Health & Environmental Excellence Center, Wilmington (Del., USA)

Year:

2003

Bibliographic source:

U.S. EPA, 46 pp

Materials and methods

Principles of method if other than guideline:

Method: other: Noakes DW and Sanderson DM (1969). Brit. J. Ind. Med. 26, 59-64.

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,5,9-Cyclododecatriene

IUPAC Name:

1,5,9-Cyclododecatriene

Constituent 2

Reference substance name:

Cyclododeca-1,5,9-triene

EC Number:

225-533-8

EC Name:

Cyclododeca-1,5,9-triene

Cas Number:

4904-61-4

Molecular formula:

C12H18

IUPAC Name:

cyclododeca-1,5,9-triene

Details on test material:

1,5,9-Cyclododecatriene, purity not specified

Test animals

Species:

rat

Strain:

other: CD

Sex:

male/female

Details on test animals or test system and environmental conditions:

age: 12-13 weeks
- housed individually over the 24-hour exposure period, during which time they were deprived of food, but allowed water ad libitum. After exposur the rats were then housed 3/cage (genders separate)

Administration / exposure

Type of coverage:

occlusive

Vehicle:

other: no vehicle

Details on dermal exposure:

ADMINISTRATION:

- Occlusion: yes
- Doses: 2, 3, or 4 ml/kg bw
-test substance was placed onto the shorn dorso-lumbar skin, and bandaged to contact the skin using an impermeable dressing of aluminum foil and waterproof plaster.
- Removal of test substance: washing with a tepid dilute detergent  solution after 24 hours

Duration of exposure:

24 h

Doses:

2, 3, or 4 ml/kg bw

No. of animals per sex per dose:

2 rats of each sex per dose

Control animals:

no

Details on study design:

-observed for signs of intoxication during the following 9 days after exposure

Statistics:

no information

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 3 520 mg/kg bw

Mortality:

no mortalities

Clinical signs:

other: All animals were seen to have eschar at the application  site on removal of the occlusive dressing.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: other: Regulation EC 1272/2008

Executive summary:

Two rats of each sex (aged 12 -13 weeks) were used at each dose level (2, 3 or 4 mL/kg). The test substance was placed onto the shorn dorso-lumbar skin, and bandaged to contact the skin using an impermeable dressing of aluminium foil and waterproof plaster. The rats were housed individually over the 24 -hour exposure period, during which time they were deprived of food, but allowed water ad libitum. After 24 hours, the dressings were removed and the exposed area was washed with a tepid dilute detergent solution. The rats were then housed 3/cage, egnders separate, and observed for signs of intoxiciation during the following 9 days. None of the rabbits died during the test. All the rats had eschar on their backs when the occlusive dressing had been removed.

Under the conditions of this study the acute toxicity of 1,5,9-cyclododecatriene after dermal application in rabbits is very low with a LD50 higher than 3520 mg/kg (4 mL/kg).