6-methyloct-5-en-2-one

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference 1

Reference Type:

other company data

Title:

Unnamed

Year:

1974

Report date:

1974

Reference 2

Reference Type:

secondary source

Title:

No information

Author:

BASF AG

Year:

2008

Bibliographic source:

RIFM database

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Principles of method if other than guideline:

Study was performed acc. internal BASF method, which was in large part equivalent to OECD 405
Deviations from OECD 405: 2 test animals; eye examination 24 hrs before testing not mentioned

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

6-methylhept-5-en-2-one

EC Number:

203-816-7

EC Name:

6-methylhept-5-en-2-one

Cas Number:

110-93-0

IUPAC Name:

6-methylhept-5-en-2-one

Details on test material:

- Name of test material (as cited in study report): 6-Methyl-5-hepten-2-one
- Analytical purity: 98 %

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: (Source: M. Gaukler, Offenbach, Germany
- Weight at study initiation: 3.04 kg (male), 2.99 kg (female)

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: left eye of same animal treated with 0.9% saline solution

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.05 ml
- Concentration (if solution): 100%

Observation period (in vivo):

Several times after application including 1 hr, 24 hrs, 72 hrs and until 8 days after application

Number of animals or in vitro replicates:

2, 1 male and 1 female

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no washing

SCORING SYSTEM:
Conjunctival redness, chemosis and corneal opacity were scored according the below described system.
Although the results in the report were originally not given as Draize scores the data can be transferred into the Draize scoring system (in brackets). (+) = none - negligible effect (Draize score 0)
+ = slight effect (1)
++ = moderate effect (2)
+++ = severe effect (> = 3)

Results and discussion

Any other information on results incl. tables

Results:

Animals: 1 - female; animal 2 - male

Readings	Animal	cornea	Iris	conjunctiva			Symptons
		opacity		redness	swelling	discharge
1h	1	1	0	1	2	0
	2	0	0	1	1	0
24 h	1	1	0	1	0	0
	2	0	0	1	0	0
8 d	1	0	0	0	0	0
	2	0	0	0	0	0

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The test substance led only to slight corneal opacity and slight conjunctival redness 24 hours after application of the test
substance into the eyes.
All effects were completely
reversible within 8 days of observation. Thus, no clssifcation for eye irritant effects necessary.