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EC number: - | CAS number: 37486-69-4
The ready biodegradability of TFEE-5 was studied in a 28-day CO2 evolution test according to OECD method 301B. Activated sludge from a domestic sewage treatment plant was used as the source of inoculum. Brown glass bottles were filled with oxygen-saturated, CO2-free medium and spiked with one of the following: TFEE-5 at 56 mg/L (12 mg TOC/L), sodium acetate (reference substance) at 40 mg/L (12 mg TOC/L), TFEE-5 plus sodium acetate (toxicity control), or inoculum alone (inoculum blank). Evolved CO2 was titrated every second or third day during the first 10 days and thereafter at least every fifth day until the 28th day for the inoculum blank and test suspension. Titrations for the positive and toxicity control were made 5 times through day 14. The 28-day biodegradation of TFEE-5 was found to be 3% by CO2 evolution. In the toxicity control more than 25% biodegradation occurred within 14 days (33%) therefore the test substance did not inhibit microbial activity. The reference substance sodium acetate showed 63% biodegradation at 14 days demonstrating the viability of the inoculum. Based on these data, TFEE-5 was found to not be readily biodegradable under the conditions of the CO2 evolution test.
This study is classified as acceptable and satisfies the guideline requirements for test method OECD301B, ready biodegradability: CO2 evolution test.
Test Organism Age: less than one day
Test Type: Open system
Percent degradability: 3% 95% C.I.: not applicable
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