3,4-dimethoxyphenylacetonitrile

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3,4-dimethoxyphenylacetonitrile

EC Number:

202-225-1

EC Name:

3,4-dimethoxyphenylacetonitrile

Cas Number:

93-17-4

Molecular formula:

C10H11NO2

IUPAC Name:

3,4-dimethoxyphenylacetonitrile

Details on test material:

- Name of test material (as cited in study report): 3,4-dimethoxyphenylacetonitrile (Veratrylcyanid)
- Lot/batch No.: 011924AX10
- Analytical purity: 98.6 %

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

The inoculum was washed and brought to a concentration of 5 g/l dry substance, then aerated during the night (24 h). 50 ml was added to the test solution total volume of 250 ml to obtain a concentration of 1 g dry substance/l in the test.
- Initial biomass concentration: 1 g/l

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test conditions

Test temperature:

20 +/- 2 °C

Nominal and measured concentrations:

Nominal test concentraions:
13.2, 100, 1000 mg/l.

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer-vessel ( 250 ml)
- Fill volume: 250 ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic medium: 8 ml/vessel 100-fold concentrated OECD medium

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

ca. 230 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

ca. 800 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

30 min

Dose descriptor:

other: EC80

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate