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Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
3,4-dimethoxyphenylacetonitrile
EC Number:
202-225-1
EC Name:
3,4-dimethoxyphenylacetonitrile
Cas Number:
93-17-4
Molecular formula:
C10H11NO2
IUPAC Name:
3,4-dimethoxyphenylacetonitrile
Details on test material:
- Name of test material (as cited in study report): 3,4-dimethoxyphenylacetonitrile (Veratrylcyanid)
- Lot/batch No.: 011924AX10
- Analytical purity: 98.6 %

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
The inoculum was washed and brought to a concentration of 5 g/l dry substance, then aerated during the night (24 h). 50 ml was added to the test solution total volume of 250 ml to obtain a concentration of 1 g dry substance/l in the test.
- Initial biomass concentration: 1 g/l

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min

Test conditions

Test temperature:
20 +/- 2 °C
Nominal and measured concentrations:
Nominal test concentraions:
13.2, 100, 1000 mg/l.
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer-vessel ( 250 ml)
- Fill volume: 250 ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic medium: 8 ml/vessel 100-fold concentrated OECD medium

Results and discussion

Effect concentrationsopen allclose all
Duration:
30 min
Dose descriptor:
other: EC20
Effect conc.:
ca. 230 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
ca. 800 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion