3,4-dimethoxyphenylacetonitrile

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3,4-dimethoxyphenylacetonitrile

EC Number:

202-225-1

EC Name:

3,4-dimethoxyphenylacetonitrile

Cas Number:

93-17-4

Molecular formula:

C10H11NO2

IUPAC Name:

3,4-dimethoxyphenylacetonitrile

Details on test material:

- Name of test material (as cited in study report): 3,4-dimethoxyphenylacetonitrile (Veratrylcyanid krist. SF9)
- Lot/batch No.: 011924AX10
- Analytical purity: 98.6 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest test concentration (100 mg/l (nominal) in M4 medium was prepared by using stirring a period of 20 min at 20 +/- 2 °C. Following this, serial dilutions of this test concentration were made in order to obtain the desired additional test concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.20 - 3.20 mmol/l

Test temperature:

19.8 - 20.4 °C

pH:

8.1 - 8.2

Dissolved oxygen:

8.5 - 9.1 mg/l

Nominal and measured concentrations:

Nominal test concentrations: 12.5, 25, 50 and 100 mg/l.

Analytical recovery rates (control analyses of 0, 12.5. 25, 50 and 100 mg/l):
after 0 h = 104 - 108 % of the nominal concentration values
after 48 h = 106 - 112 % of the nominal concentration values

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium (according to ISO 10706)
- Alkalinity up to pH 4.3: 0.80 - 1.00 mmol/l
- Ca/mg ratio: 4 : 1
- Conductivity: 616 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime
- Light intensity: diffuse light, 2 - 7 microE/(m2*s) at a wave length of 400 - 700 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 hours

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

The EC 50 was determined directly from the raw data

Any other information on results incl. tables

Percentage of daphnia Immobility after x hours: 
       
Nominal 
test 
concentration
 (mg/l)    /   0 h /  24 h /  48 h /
Control    /   0   /   0   /   0   / 
12.5       /   0   /   0   /   0   /
25         /   0   /   0   /   0   /
50         /   0   /   0   /   0   /
100        /   0   /   0   /   0   /