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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
3,4-dimethoxyphenylacetonitrile
EC Number:
202-225-1
EC Name:
3,4-dimethoxyphenylacetonitrile
Cas Number:
93-17-4
Molecular formula:
C10H11NO2
IUPAC Name:
3,4-dimethoxyphenylacetonitrile
Details on test material:
- Name of test material (as cited in study report): 3,4-dimethoxyphenylacetonitrile (Veratrylcyanid krist. SF9)
- Lot/batch No.: 011924AX10
- Analytical purity: 98.6 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest test concentration (100 mg/l (nominal) in M4 medium was prepared by using stirring a period of 20 min at 20 +/- 2 °C. Following this, serial dilutions of this test concentration were made in order to obtain the desired additional test concentrations.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.20 - 3.20 mmol/l
Test temperature:
19.8 - 20.4 °C
pH:
8.1 - 8.2
Dissolved oxygen:
8.5 - 9.1 mg/l
Nominal and measured concentrations:
Nominal test concentrations: 12.5, 25, 50 and 100 mg/l.

Analytical recovery rates (control analyses of 0, 12.5. 25, 50 and 100 mg/l):
after 0 h = 104 - 108 % of the nominal concentration values
after 48 h = 106 - 112 % of the nominal concentration values
Details on test conditions:
TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium (according to ISO 10706)
- Alkalinity up to pH 4.3: 0.80 - 1.00 mmol/l
- Ca/mg ratio: 4 : 1
- Conductivity: 616 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime
- Light intensity: diffuse light, 2 - 7 microE/(m2*s) at a wave length of 400 - 700 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 hours

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Reported statistics and error estimates:
The EC 50 was determined directly from the raw data

Any other information on results incl. tables

Percentage of daphnia Immobility after x hours: 

Nominal
test
concentration
(mg/l) / 0 h / 24 h / 48 h /
Control / 0 / 0 / 0 /
12.5 / 0 / 0 / 0 /
25 / 0 / 0 / 0 /
50 / 0 / 0 / 0 /
100 / 0 / 0 / 0 /


Applicant's summary and conclusion