[No public or meaningful name is available]

Repeated dose toxicity: dermal

Administrative data

Endpoint:

short-term repeated dose toxicity: dermal

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: no guideline study but menteiond in peer-reviewed publication

Data source

Reference

Reference 1

Title:

Unnamed

Year:

1968

Reference 2

Reference Type:

secondary source

Title:

1,4-Dichlor-2-nitrobenzol

Author:

BUA

Year:

1991

Bibliographic source:

BUA-Stoffbericht 65, ISBN 3-527-28462-1, VChVerlagsgesellschaft mbH, 8940 Weinheim

Materials and methods

Test guideline

Guideline:

other: guideline not mentioned

Principles of method if other than guideline:

once daily for 15 days dermal application of different doses to the shaved back of female rabbits

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,4-dichloro-2-nitrobenzene

EC Number:

201-923-3

EC Name:

1,4-dichloro-2-nitrobenzene

Cas Number:

89-61-2

Molecular formula:

C6H3Cl2NO2

IUPAC Name:

1,4-dichloro-2-nitrobenzene

Test material form:

other: solid

Details on test material:

no data

Test animals

Species:

rabbit

Strain:

other:

Sex:

female

Details on test animals or test system and environmental conditions:

no data

Administration / exposure

Type of coverage:

not specified

Vehicle:

not specified

Details on exposure:

once daily dermal application of different doses to the shaved back of groups of 5 female rabbits

Analytical verification of doses or concentrations:

not specified

Details on analytical verification of doses or concentrations:

no data

Duration of treatment / exposure:

up to 15 days (see dosing scheme in details on study design)

Frequency of treatment:

once daily

Doses / concentrations

Remarks:

Doses / Concentrations:
100, 200, 400 mg/kg bw/day
Basis:

No. of animals per sex per dose:

up to 5 females per dose see dosing scheme in details on study design

Control animals:

not specified

Details on study design:

100 mg/kg bw was applied to 5 animals on 15 days
200 mg/kg bw was applied to 5 animals on 3 days
200 mg/kg bw was applied to 5 animals on 15 days
400 mg/kg bw was applied to 5 animals on 10 days
400 mg/kg bw was applied to 5 animals on 11 days
400 mg/kg bw was applied to 5 animals on 12 days

Positive control:

no data

Examinations

Observations and examinations performed and frequency:

molrality clinidal signs and hematology

Sacrifice and pathology:

macroscopic evaluation

Statistics:

no data

Results and discussion

Results of examinations

Details on results:

Mortality:
100 mg/kg bw was applied to 5 animals on 15 days: 0/5
200 mg/kg bw was applied to 5 animals on 3 days: 1/5
200 mg/kg bw was applied to 5 animals on 15 days 1/5
400 mg/kg bw was applied to 5 animals on 10 days: 5/5
400 mg/kg bw was applied to 5 animals on 11 days: 5/5
400 mg/kg bw was applied to 5 animals on 12 days: 5/5

dose depending decreae of erythrocytes and hemoglobin; urinary discoloration
no histopathololgical changes in heart lung kidney adremal liver
spleen of the animals dosed with 400 mg/kg bw showed hyperemy,deposition of pigment and increased erythropoiesis

Effect levels

1

Dose descriptor:

NOAEL

Remarks on result:

not determinable

Remarks:

no NOAEL identified

2

Dose descriptor:

LOAEL

Effect level:

100 mg/kg bw/day

Sex:

female

Basis for effect level:

other: based on dose -depending impairment of blood picture (no details)

Target system / organ toxicity

Critical effects observed:

not specified