Reaction mass of 2-Piperazin-1-ylethanol and Piperazine-1,4-diethanol and Piperazine

Short-term toxicity to fish

Description of key information

With high probability acutely not harmful to fish.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

870 mg/L

Additional information

There is one reliable study available regarding the toxicity of the reaction mass of 2-piperazin-1-ylethanol and piperazine-1,4-diethanol and piperazine. The toxic effect of a hydroxyethylpiperazine mixture was studied according to German Industrial Standard DIN 38412, part 15 under static conditions. The test species was Leuciscus idus. The test item concentrations were not analyticaly verified, but are assumed to be stable over the exposure period. The 96 -h LC50 was determined to be ca. 870 mg/L based on not pH-adjusted test solutions. A pH-adjusted test sample of 1000 mg/L caused no mortality (BASF AG, 1989; report no. 10F0949/885065).

The low acute toxicity of the reaction mass to fish is supported by the experimental data available for the major components of the reaction mass:

The toxic effect of 2-piperazin-1-ylethanol (CAS 103-76-4) was studied according to German Industrial Standard DIN 38412, part 15 under static conditions. The test species was Leuciscus idus. The test item concentrations were not analyticaly verified. The 96-h LC50 was determined to be > 464 and < 1000 mg/L based on not pH-adjusted test solutions. A pH-adjusted test sample of 4640 mg/L caused no mortality (BASF AG, 1988; report no. 10F0724/875016).

This finding is supported by a study with the test species Pimephales promelas under flow-through conditions performed similar to OECD 203 (Geiger et al., 1986). The 96-h LC50 was determined to be 6410 mg/L using pH-adjusted test solutions. The test item concentrations were analytically verified.

Piperazine-1,4-diethanol (CAS 122-96-3) was tested in a static test according to German Industrial Standard DIN 38412, part 15 with Leuciscus idus as test species. The test material was a solution containing 48% piperazin-1,4-diethanol (CAS 122 -96 -3), 14% 2-piperazin-1-ylethanol (CAS 103-76-4) and 0.6% piperazine (CAS 110 -85 -0). The test item concentrations were not analytically verified, but are assumed to be stable. The 96 -h LC50 based on the test matrerial was >2150 and < 4640 mg/L. The test results were recalculated to give an effect value based on piperazin-1,4-diethanol alone. The 96-h LC50 was determined to be > 1040 and < 2246 mg/L. A pH-adjusted test sample of 4640 mg/L caused no mortality (BASF AG, 1990; report no. 10F0292/895204).

The toxic effect of piperazine (CAS 110 -85 -0) on Poecilia reticulata was studies according to EU method C.1 in a semi-static test without analyticl monitoring. The test cocnentrations were assumed to be stable over the exposure period of 96 h. The 96 -h LC50 was determined to be greater than 1800 mg/L based on pH-adjusted test solutions. In addition the toxic effect on the marine fish species Cyprinodon variegatus was studied in a semi-static acute toxicity test according to OSPAR Commission 2006, part B. The test item concentrations were not analytically verified but are assumed to be stable. The limit test showed no mortality at a concentration of 266 mg/L after 96 h (96-h LC0 = 266 mg/L; BASF SE, 2011; report no. 1481b-22).

In conclusion, the "reaction mass of 2 -piperazin-1 -ylethanol, piperazine-1,4 -diethanol, piperazine" is assessed to be with high probability acutely not harmful to fish.