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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The substance is non-readily biodegradable. 

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

Biodegradation test of test substance by micro-organisms:

Conditions of cultivation:

(1) Concentration of test substance: 100 mg/L

(2) Concentration of activated sludge (as the concentration of suspended solid): 30 mg/L

(3) Volume of test solution: 300 mL

(4) Cultivation temperature: 25 ± 1°C

(5) Cultivation duration: 28 days

Measurement and analysis:

(1) Measurement of biochemical oxygen demand (BOD) with a closed system oxygen consumption measuring apparatus.

(2) Determination of test substance by high performance liquid chromatography (HPLC).


(1) Percentage biodegradation by BOD: 0%, 0%, 0%; average 0%

(2) Percentage biodegradation by HPLC: 5%, 0%, 0%; average 2%


The test substance was not biodegraded by the micro-organisms under the test conditions.