Octane, 1,1,1,2,2,3,3,4,4,5,5,6,6-tridecafluoro-

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP compliant guideline study, available as unpublished report, no restrictions, fully adequate for assessment.

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

An initial loading rate of 200 mg/L was stirred fir a period of at least 24 hours in a closed test bottle. Subsequently, synthetic sewage feed and sludge were added resulting in a final loading of 100 mg/L.

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

Sludge originated from the municipal sewage treatment plant "Waterschap de Maaskant in Den Bosch, the Netherlands".

Upon retrieval the sludge was coarsely sieved and diluted with ISO-medium. The amount of suspended solids was 3.9 g/L.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

3 h

Test temperature:

18.1 to 18.9 ºC

pH:

8.6

Nominal and measured concentrations:

Nominal: 100 mg/L

Details on test conditions:

TEST SYSTEM
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass, 1 L
- Aeration: yes
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

- Effect concentrations exceeding solubility of substance in test medium: yes

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Relevant effect levels: 20.1 mg/L

Validity criteria fulfilled:

yes

Conclusions:

The 3-h EC50 value of AC-6000 in domestic activated sludge is >100 mg/L.

Executive summary:

In a GLP-compliant OECD 209 guideline study for AC-6000with predominantly domestic sludge, activated sludge (0.7 g/L suspended solids, 2 replicates) was exposed to a nominal concentration of 100 mg/L for 3 hours.




The test concentration was prepared by stirring the test solution with a target dose level of 200 mg/L for 24 hours before exposure. The growth inhibition rate of the test substance was 9%. The 3-h EC50 value is >100 mg/L, this effect concentration is larger than the water solubility.

Description of key information

The 3-h EC50 value of AC-6000 in domestic activated sludge is >100 mg/L; this value will be used in the assessment.

Key value for chemical safety assessment

EC50 for microorganisms:

100 mg/L

Additional information

A GLP-compliant OECD 209 guideline study with predominantly domestic sludge is available for AC-6000 (NOTOX B.V., 2007). Activated sludge (0.7 g/L suspended solids, 2 replicates) was exposed to a nominal concentration of 100 mg/L for 3 hours.

The test concentration was prepared by stirring the test solution with a target dose level of 200 mg/L for 24 hours before exposure. The growth inhibition rate of the test substance was 9%. The 3-h EC50 value is >100 mg/L, this effect concentration is larger than the water solubility.