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Toxicity to aquatic algae and cyanobacteria

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Description of key information

The 72-h EC50 value and the 72-h NOEC of AC-6000 in Pseudokirchneriella subcapitata are >0.072 mg/L and ≥0.072 mg/L, respectively; these values will be used in the assessment.

Key value for chemical safety assessment

Additional information

A GLP-compliant OECD 201 guideline study with Pseudokrichneriella subcapitata is available for AC-6000 (Kurume Laboratory, 2007c). Algae (5E03 cells/mL, six replicates) were exposed to a nominal concentration of 100 mg/L for 72 hours. The test concentration was analytically verified by GC-MS from two additional vessels prepared for analytical chemistry, and was less then 80% of nominal concentrations.

The test concentration was prepared by stirring the test solution with a target dose level of 100 mg/L for 48 hours before exposure. As the measured concentration at 24 hours was much lower than the measured concentration at 48 hours, it can be assumed that something was wrong with the analyses at 24 hours. This value was therefore omitted from calculating the geometric mean concentration of the test substance.

 The growth inhibition rate of the test substance was -3.36%. The EL50 value is >100 mg/L, the corresponding 48-h EC50 value >0.072 mg/L,this effect concentration is larger than the water solubility.