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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study conducted in accordance with "Good Scientific Practice"

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report date:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
no
Remarks:
but in accordance with the principles of "Good Scientific Practice"

Test material

Constituent 1
Chemical structure
Reference substance name:
4-amino-2,5-dichloro-N,N-dimethylbenzenesulphonamide
EC Number:
247-501-2
EC Name:
4-amino-2,5-dichloro-N,N-dimethylbenzenesulphonamide
Cas Number:
26175-68-8
Molecular formula:
C8H10Cl2N2O2S
IUPAC Name:
4-amino-2,5-dichloro-N,N-dimethylbenzene-1-sulfonamide

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Poecilia reticulata
Details on test organisms:
Breeding: Parental and young fish population was held in two 250 l glass aquaria
Test vessel: 1 l glass aquariuq
Test medium: Tap water from Zürci non aerated
Number of fish: 7 fish per test concentration
Fish length: 1 - 3 cm
Light: 14 hour photoperiod a day supplied by overhead white fluorescent tubes
Temperatute: 22+-1 °C
Feed: Sera micropan; Sera vipan (Sera apanristic GmbH, D-5138 Heidelberg)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
1.61 - 1.72 mmol/L (total hardness)
Test temperature:
20.9 - 22.2 °C
pH:
7.6 - 8.3
Dissolved oxygen:
7.0 - 8.3 mg/l
Nominal and measured concentrations:
Nominal: 1.52, 2.74, 4.94, 8.88, 16.0 mg/l
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
other: cumulative mortality 1/7
Effect conc.:
1.52 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: cumulative mortality 0/7
Effect conc.:
2.74 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: cumulative mortality 0/7
Effect conc.:
4.94 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: cumulative mortality 1/7
Effect conc.:
8.88 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
other: cumulative mortality 6/7
Effect conc.:
16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The LC50 was determined as 13.4 mg/l
Executive summary:

The acute lethal toxicity of ADCSF to Poecilia reticulata (Guppy) was investigated under static exposure conditions over a period of 96 hours. The nominal concentrations were 1.52, 2.74, 4.94, 8.88 and 16.0 mg/l. Mortality was observed after 96 hours (cumulated) at 16.0 mg/l (6/7), 8.88 mg/l (1/7) and 1.52 mg/l (1/7).

Based on the data the LC50 was calculated to be 13.4 mg/l