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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Jan - Apr 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: According to OECD guideline under GLP
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997
Report Date:
1997

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
Principles of method if other than guideline:
not relevant
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 1,4,6-Trienol
- Analytical purity: 97%
- Lot/batch No.: 25024063

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
> 4.4 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
> 4.4 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
biomass

Any other information on results incl. tables

Table 1: Inhibition of the growth of Scenedesmus subspicatus after 96 hours exposure to 1,4,6 -Trienol

 Dilution    Inhibition in percent  
 of saturated solution    Biomass (integral)    Growth rate  
 Control   0 0
 1:100   46,9 9,7
 1:10   0,5 1,5
 Saturated solution   4,7 1,4

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
1,4,6-Trienol had no inhibitory effect on the growth of the Scenedesmus subspicatus. It is not
known why the lowest concentration showed a reduced growth. However, since the highest
concentration showed no effect, it is not considered to be substance-related.
Executive summary:

The purpose of this study was to determine the effects of the test compound 1,4,6 -Trienol on the growth of the green algae Scenedesmus subspicatus. The study was conducted in agreement with the OECD guideline no. 201.

The test substance was incubated in all aqueous solution including nutrients with an algae population of Scenedesmus subspicatus for a test duration of approximately 96 hours. The incubation period was extended to 96 hours, since the cell concentration was initially lower than recommended by the guideline. The nutrient solution was made up of mainly nitrc:lte, phosphates and some trace elements. The algae were incubated in an incubator shaker under continuous light. The test concentrations were a saturated solution and dilutions of 1: 10 and 1: 100. The algae were exposed to each concentration in triplicates. Six vessels were prepared for the control. The concentration of a saturated solution of 1,4,6 -Trienol was analysed by a TOC analyzer and calculated based on the molecular fomula. It was estimated with 4.4 mg/I.

1,4,6 -Trienol had no inhibitory effect on the growth of the Scenedesmus subspicatus.