Sodium p-(3-amino-4-methylbenzamido)benzenesulphonate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994- 1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well performed OECD study but only weak documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Sodium p-(3-amino-4-methylbenzamido)benzenesulphonate

EC Number:

275-177-2

EC Name:

Sodium p-(3-amino-4-methylbenzamido)benzenesulphonate

Cas Number:

71077-34-4

Molecular formula:

C14H14N2O4S.Na

IUPAC Name:

sodium 4-[(3-amino-4-methylbenzoyl)amino]benzenesulfonate

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

from waste water treatment plant Frankfurt_Sindlingen, washed, centrifuged and resuspended.
6 days aeration to eliminate the carbon contained in the sludge
2.2 ml/L sludge with 0.11 g/L of the centrifuged sediment was used.
dry content 9.062 g/L identical with 20 mg/L dry content in the endvolume of the test batch
sludge is not adapted to the test item

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

1 g/L

Based on:

test mat.

Details on study design:

Shaking machine with about 100 rpm
Temperature 21-22°C

Reference substance

Reference substance:

benzoic acid, sodium salt

Remarks:

concentration: 20 mg/L (DOC)

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

1 d

% Degradation 2

Parameter:

% degradation (DOC removal)

Value:

1.3

Sampling time:

4 d

% Degradation 3

Parameter:

% degradation (DOC removal)

Value:

10.3

Sampling time:

7 d

% Degradation 4

Parameter:

% degradation (DOC removal)

Value:

7.3

Sampling time:

15 d

% Degradation 5

Parameter:

% degradation (DOC removal)

Value:

13.5

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

The substance has to be considered as not readily biodegradable.

Executive summary:

A test fornready biodegradation was performed with the substance according OECD 301 A. Under test conditions a biodegradation of 13.5% after 28 days was observed. The reference substance sodium benzoate reached complete degradation after 7 days.