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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994- 1995
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: well performed OECD study but only weak documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
Qualifier:
according to guideline
Guideline:
EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium p-(3-amino-4-methylbenzamido)benzenesulphonate
EC Number:
275-177-2
EC Name:
Sodium p-(3-amino-4-methylbenzamido)benzenesulphonate
Cas Number:
71077-34-4
Molecular formula:
C14H14N2O4S.Na
IUPAC Name:
sodium 4-[(3-amino-4-methylbenzoyl)amino]benzenesulfonate

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
from waste water treatment plant Frankfurt_Sindlingen, washed, centrifuged and resuspended.
6 days aeration to eliminate the carbon contained in the sludge
2.2 ml/L sludge with 0.11 g/L of the centrifuged sediment was used.
dry content 9.062 g/L identical with 20 mg/L dry content in the endvolume of the test batch
sludge is not adapted to the test item
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
1 g/L
Based on:
test mat.
Details on study design:
Shaking machine with about 100 rpm
Temperature 21-22°C
Reference substance
Reference substance:
benzoic acid, sodium salt
Remarks:
concentration: 20 mg/L (DOC)

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
1 d
Parameter:
% degradation (DOC removal)
Value:
1.3
Sampling time:
4 d
Parameter:
% degradation (DOC removal)
Value:
10.3
Sampling time:
7 d
Parameter:
% degradation (DOC removal)
Value:
7.3
Sampling time:
15 d
Parameter:
% degradation (DOC removal)
Value:
13.5
Sampling time:
28 d

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other: not readily biodegradable
Conclusions:
The substance has to be considered as not readily biodegradable.
Executive summary:

A test fornready biodegradation was performed with the substance according OECD 301 A. Under test conditions a biodegradation of 13.5% after 28 days was observed. The reference substance sodium benzoate reached complete degradation after 7 days.