Zirconium tetranitrate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP non-guideline animal experimental study, limitations in design and/or reporting but nevertheless adequate for assessment

Data source

Reference

Reference Type:

publication

Title:

Acute toxicity of zirconium, columbium, strontium, lethanum, cesium, tantalum and yttrium.

Author:

Cochran, K.W., Doull, J., Mazur, M. and DuBois, K.P.

Year:

1950

Bibliographic source:

Arch. Ind. Hyg. Occ. Med., 1(6), 637-650 (1950)

Materials and methods

Principles of method if other than guideline:

In this acute oral toxicity study 24 adult Sprague Dawley rats (both sexes) were used. The animals received single 50% aqueous zirconyl acetate solutions orally by stomach tube. All animals were observed for ten days, but initial groups receiving a given compound were kept for thirty days to ascertain whether any significant mortality occurred after the tenth day. The LD50 values were obtained from ten day mortality data by using the log-probability method.

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Zirconium di(acetate) oxide

EC Number:

225-924-3

EC Name:

Zirconium di(acetate) oxide

Cas Number:

5153-24-2

IUPAC Name:

5153-24-2

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: adult rats
- Weight at study initiation: 200-300 gram
- Diet: ad libitum
- Water: ad libitum

ENVIRONMENTAL CONDITIONS
animals were maintained in air-conditioned rooms

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 50% aqueous solution

Doses:

No details given.

No. of animals per sex per dose:

24 rats (no details on the number of males and females)

Control animals:

no

Details on study design:

- Duration of observation period following administration: all animals were observed for 10 days, but initial groups receiving a given compound were kept for 30 days to ascertain whether any significant mortality occurred after the 10th day. It was found that 10-day observation periods were sufficient since animals either succumbed or recovered during this period.
- Other examinations performed: physiology and gross pathology

Statistics:

The LD50 values were obtained from ten day mortality data by using the log-probability method.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

4 100 mg/kg bw

Based on:

test mat.

Remarks on result:

other: No sex differences were noted in the degree to which rats were susceptible to the substance investigated. LD50 values were therefore derived from the combined data on both sexes.

Mortality:

The time of death varied from a few hours to a few days following the administration of the compound. Few deaths however occurred later than five days after administration of zirconium salts.

Clinical signs:

other: Progressive depression and decrease in activity until death occurred.

Gross pathology:

No characteristic gross pathologic changes were observed.