Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Scientifically acceptable publication; however, the exposure period was reduced to only 90 minutes. Thus, there is not enough confidence to estimate the hazard potential to fish.

Data source

Reference
Reference Type:
publication
Title:
Biological effects of prenylated hydroquinones: structure-activity relationship studies in antimicrobial, brine shrimp, and fish lethality assays
Author:
De Rosa, S., De Giulio, A. and Iodice, C.
Year:
1994
Bibliographic source:
Journal of Natural Products, Vol. 57, No. 12, pp. 1711-1716

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
A test guideline was not mentioned in the publication.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylhydroquinone
EC Number:
202-443-7
EC Name:
2-methylhydroquinone
Cas Number:
95-71-6
Molecular formula:
C7H8O2
IUPAC Name:
2-methylbenzene-1,4-diol
Constituent 2
Reference substance name:
Methylhydroquinone
IUPAC Name:
Methylhydroquinone
Details on test material:
- Origin of test material: Sigma-Aldrich s.r.l., Italy

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION OF TEST SOLUTION
- Test item was dissolved in Me2CO (1% final volume)

Test organisms

Test organisms (species):
Gambusia affinis
Details on test organisms:
- Common name: mosquito fish
- Weight of fish: 0.1 - 0.3 g

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
90 min

Test conditions

Nominal and measured concentrations:
- Concentrations tested (nominal): 100, 10, 1, 0.1, 0.01 and 0.001 ppm plus control
Details on test conditions:
TEST SYSTEM
- Replicates per concentration: 3
- Replicated per control: 2
- No. of fish per concentration: 6
- Introduction of organism to test medium: fish were placed in 70 mL of distilled H2O to which appropriate amounts of compound (dissolved in Me2CO) were added.

Results and discussion

Effect concentrations
Duration:
90 min
Dose descriptor:
LC50
Effect conc.:
38.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95% C.L.: 20.2-76.8 mg/L
Reported statistics and error estimates:
The data was statistically analyzed by the Finney program.

Applicant's summary and conclusion