Isopentylamine

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15. Oct 1987 - 29. Oct 1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented report which meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Principles of method if other than guideline:

BASF-Test: The study was conducted according to an internal BASF method which in principle is comparable to the OECD Guideline 401.
A test group consisting of 5 animals/sex was treated by single gavage application with an aqueous solution of the test substance. The animals were observed for mortality and for clinical symptoms of toxicity. At the end of the observation period of 14 days, the surviving animals were sacrificed for the purpose of necropsy; animals that died during the observations period also were subjected to necropsy.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Isopentylamine

EC Number:

203-526-0

EC Name:

Isopentylamine

Cas Number:

107-85-7

Molecular formula:

C5H13N

IUPAC Name:

isopentylamine

Details on test material:

- Name of test material (as cited in study report): Isopentylamin

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: DR. K. THOMAE GMBH, Biberach, Germany
- Weight at study initiation: male: 191 g (mean); female: 189 g (mean).
- Fasting period before study: 16 h
- Housing: 5 animals per cage
- Diet: KLIBA-LABORDIAET 343, Klingentalmuehle AG, Switzerland, ad libitum
- Water: tap water ad libitum
- Acclimation period: at least 1 week


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24 °C
- Humidity (%): 30-70 %
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 2.15%, 4.64% or 10%
- Amount of vehicle (if gavage): 10 ml

Doses:

215, 464 or 1000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: several times on the day of administration, at least once each workday.
- Frequency of weighing: dasy 0, 7 and 13
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

341 mg/kg bw

95% CL:

251 - 460

Mortality:

1000 mg/kg: 1 female died within 1 h. By 24 h all animals were dead.
464 mg/kg: 4 males and females each died within 24 h.
215 mg/kg: 1 female died within 13 days.

Clinical signs:

1000 mg/kg: After 30 min up to 1 h the animals showed dyspnea, apathy, abnormal position, staggering, piloerection, cyanosis, poor general state (see details in table in remarks on results).
464 mg/kg: dyspnea, apathy, piloerection, poor general state, staggering, abnormal position, spastic gait, exopthalmus, impaired general state (see details in table in remarks on results).

Body weight:

see detailed table in remarks on results.

Gross pathology:

Animals that died:
General congestion. Stomach/small intestine: mucosa reddened.
In one animal which died 13 days after substance administration cachexia and empty intestines were noted.

Any other information on results incl. tables

Mortality:

Dose (mg/kg )	Conc.	Gender	1 h	24 h	48 h	day 7	day 14
1000	10	male	0/5	5/5	5/5	5/5	5/5
1000	10	female	1/5	5/5	5/5	5/5	5/5
464	4.64	male	0/5	4/5	4/5	4/5	4/5
464	4.64	female	0/5	4/5	4/5	4/5	4/5
215	2.15	male	0/5	0/5	0/5	0/5	0/5
215	2.15	female	0/5	0/5	0/5	0/5	1/5

Weight (g):

Dose (mg/kg)	Gender	day 0	day 7	day 13
1000	male	198	-	-
1000	female	197	-	-
464	male	183	163	189
464	female	182	199	208
215	male	191	243	278
215	female	189	211	209

Clinical signs:

Dose (mg/kg)	1000		464
	male	female	male	female
Dyspnea	30 min - 1 h	30 min - 1 h	30 min - 12 d	30 min - 5 d
Apathy	30 min - 1 h	30 min - 1 h	30 min - 8 d	30 min - 5 d
Abnormal position	1 h	1 h	1 h - 4 h	1 h - 5 h
Staggering	1 h	1 h	1 h - 11 d	1 h - 5 d
Spastic gait	-	-	1 d - 5 d	1 d - 4 d
Piloerection	30 min - 1 h	30 min - 1 h	30 min - 11 d	30 min - 5 d
Exopthalmus	-	-	4 h - 5 h	-
Cyanosis	-	1 h	-	-
Impaired general state	-	-	11 d	-
Poor general state	30 min - 1 h	30 min - 1 h	30 min - 8 d	30 min - 5 d

min: minutes

h: hour

d: day

There is indication that the test substance causes local irritation to exposed tissue.

Harmful if swallowed.