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EC number: 700-720-1 | CAS number: -
Earthworm Mortality in [%] after 7 and 14 Days of Exposure
[mg/kg soil dry weight]
No earthworm showed any symptoms or behavioural changes in the control as well as in the limit test item concentration of 1000 mg/kg soil dry weight.
Earthworm Behaviour and Pathological Symptoms after 7 and 14 Days of Exposure
a) no obvious pathological symptoms or behavioural changes
Mean Biomass and Mean Loss of Body Weight
Mean live weight
Comparison versus Control
MV ± SD
0.399 ± 0.04
0.372 ± 0.05
0.399 ± 0.03
0.362 ± 0.04
Results with the reference item: The acute toxicity of 2-Chloroacetamide on the earthwormEisenia fetida fetida(SAvIGNY) wasdetermined over aperiod of two weeks from 2009-02-12 to 2009-02-26. The LC50-value ranged between 20 and 80 mg/kg soil dry weight as recommended by the guideline.
NOEC and LC-Values after 14 d for 2-Chloroacetamide
Concentration[mglkg soil dry weight]
95%-Confidence Interval[mg/kg soll dry weight]
45.8 - 55.5
The acute effects of Licowax OP on the earthworm Eisenia fetida were determined according to the OECD Guideline 207 and DIN ISO 11268-1 at Dr.U.Noack-Laboratorien in 31157 Sarstedt, Germany from July 27th to August 11th, 2009. The study was conducted with a limit test item concentration of 1000 mg/kg soil dry weight throughout 14 days. Artificial soil without test item was used as control. 40 test organisms with an individual weight between 0.3 - 0.6 g, divided into four replicates, were tested at the limit test item concentration and control in artificial soil containing 10 % peat.
No mortality as well as no pathological symptoms or changes in the earthworm behaviour were observed at the limit test item concentration of 1000 mg/kg soil dry weight after 14 days of exposure (Table 1). Compared to the control, the biomass of the earthworms at the limit test item concentration of 1000 mg/kg soil dry weight was not statistically significantly reduced at test end.
Consequently, the no observed effect concentration (NOEC) with respect to mortality, loss of biomass and other symptoms was determined to be
at 1000 mg/kg soil dry weight.
All validity criteria of the test guideline were fulfilled.
Summary of all Effects
Loss of body weight
Behaviour and pathological symptoms
Mortality, loss of body weight, other symptoms
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