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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Accetable, well-documented publication

Data source

Reference
Reference Type:
publication
Title:
CD36 mediates both cellular uptake of very long chain fatty acids and their intestinal absorption in mice
Author:
Drover VA, Nguyen DV, Bastie CC, Darlington YF, Abumrad NA, Pessin JE, London E, Sahoo D, Phillips MC
Year:
2008
Bibliographic source:
Journal of Biological Chemistry, 283: 13108-13115
Report date:
2008

Materials and methods

Objective of study:
toxicokinetics
Principles of method if other than guideline:
Study on gastrointestinal uptake of long chain fatty acids (C16-C26)
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
very long-chain fatty acids
IUPAC Name:
very long-chain fatty acids
Radiolabelling:
yes
Remarks:
14C

Test animals

Species:
mouse
Strain:
other: C57BL6/J and derived knockout strain CD36-/-
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
corn oil
Duration and frequency of treatment / exposure:
single exposure
No. of animals per sex per dose / concentration:
5-8
Control animals:
no

Results and discussion

Toxicokinetic / pharmacokinetic studies

Details on absorption:
Absorption efficiency in wild type animals: about 85% for hexadecanoic acid, about 50% for tetracosanoic acid (estimated from figure)
CD36-/- mice showed a comparable uptake of hexadecanoic acid, but only 1.3% uptake of tetracosanoic acid
The intestinal absorption was lower for the tetracosanoic acid (but not for hexadecanoic acid) in animals with a high fat, high cholesterol diet compared to standard chow.

Metabolite characterisation studies

Metabolites identified:
no

Applicant's summary and conclusion

Conclusions:
CD36 is a specific transport system in the mouse intestine, facilitating the uptake of very long chain fatty acids.
Executive summary:

Wild type mice, fed with a high fat, high cholesterol diet showed an absorption efficiency of about 85% for hexadecanoic acid and about 50% for tetracosanoic acid (estimated from figure). CD36-/- mice had a comparable uptake of hexadecanoic acid, but only 1.3% uptake of tetracosanoic acid.

The intestinal absorption was lower for the tetracosanoic acid (but not for hexadecanoic acid) in animals with a high fat, high cholesterol diet compared to standard chow.