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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
other: Body responsible for the test
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: EC B. 14/Ames test
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
A mixture of: 2,2,6,6-tetramethylpiperidin-4-yl-hexadecanoate; 2,2,6,6-tetramethylpiperidin-4-yl-octadecanoate
EC Number:
415-430-8
EC Name:
A mixture of: 2,2,6,6-tetramethylpiperidin-4-yl-hexadecanoate; 2,2,6,6-tetramethylpiperidin-4-yl-octadecanoate
Cas Number:
86403-32-9
Molecular formula:
C25H49NO2; C27H53NO2
IUPAC Name:
Reaction mass of 2,2,6,6-tetramethylpiperidin-4-yl hexadecanoate and 2,2,6,6-tetramethylpiperidin-4-yl octadecanoate

Method

Species / strain
Species / strain / cell type:
bacteria, other: Salmonellatyphimurium LT 2TA 98 TA 100 TA 1535 TA 1537
Metabolic activation system:
S9-Mix
Test concentrations with justification for top dose:
Concentration range in the main test (with metabolic activation): 8... 5000 μg/plate
Concentration range in the main test (without metabolic activation): 8... 5000 μg/plate
Vehicle / solvent:
Solvent: Ethanol
Details on test system and experimental conditions:
Concentration of the test substance resulting in precipitation: 1000 μg/plate

Results and discussion

Test resultsopen allclose all
Species / strain:
other: as specified above
Metabolic activation:
with
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
(32 μg/plate)
Species / strain:
other: as specified above
Metabolic activation:
with
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
(1000 μg/plate)
Species / strain:
other: as specified above
Metabolic activation:
without
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
(40 μg/plate)
Remarks on result:
other: other: preliminary test
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

Commens:

Concentration of the test substance observed to be toxic to bacteria;

a) in the preliminary test

- with metabolic activation: 32- 10000 μg/plate

b) in the main test

- with metabolic activation; 1000 -5000 μg/plate

-without meatabolic activation: 40 -500 μg/plate

(The strains show different sensitivity)

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation

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