1H,3H-Naphtho[1,8-cd]pyran-6,7-dicarboxylic acid, 1,3-dioxo-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study according to OECD Guideline No. 203

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,4-dioxo-3-oxatricyclo[7.3.1.0⁵,¹³]trideca-1(13),5,7,9,11-pentaene-8,10-dicarboxylic acid

EC Number:

619-817-4

Cas Number:

52671-72-4

Molecular formula:

C14H6O7

IUPAC Name:

2,4-dioxo-3-oxatricyclo[7.3.1.0⁵,¹³]trideca-1(13),5,7,9,11-pentaene-8,10-dicarboxylic acid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION OF TEST SOLUTION

The test substance was intensively stirred for 5 min with dilution water and the mixture was incorporated into the test vessel and stirred for another 2 hours. Complete dissolution was achieved, but 48 h after starting of the test precipitate on the bottom of the test vessel was observed.
After dissolving the test substance the pH of 4.0 was adjusted to 8.1 with 9.25 mL of saturated sodium hydroxide solution. During the study the pH again decreased to pH 6.2 -6.5.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebrafish
- Strain: Brachydanio rerio (Hamilton-Buchanan)
- Source: Hoechst AG, breeding colony
- Age at study initiation: 6 months
- Length at study initiation (mean, range and SD): 2.8 cm, 2.5 - 3.1 cm, SD 0.19
- Feeding during test: No feeding during the test

ACCLIMATION
- Acclimation period: at least 14 days
- Type and amount of food: Tetra Min, ad libitum
- Feeding frequency: 2 times per day

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 1°C
- Photoperiod (hrs dark / hrs light): 12/12
- The dissolved oxygen concentration was more than 80 % of the air saturation value

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

Observations were carried out during exposure at the beginning of the experiment and after 2 - 4, 24, 48, 72 and 96 hours.

Test conditions

Test temperature:

22.3°C - 22.4°C (treatment), 21.9°C - 22.3°C (control)

pH:

6.1 - 8.1 (treatment), 7.9 - 8.2 (control)

Dissolved oxygen:

7.8 - 8.7 mg/L (treatment); 7.6 - 8.9 mg/L (control)

Nominal and measured concentrations:

Nominal concentration: 500 mg/L
Highest test concentration according to recommendation of the German Federal Ministry for the Environment, Nature conservation and Nuclear Safety for ecotoxicological test procedures.

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass-aquaria 30 cm x 22 cm x 24 cm, 10 L per vessel
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: fish density in the tanks was less than 1 g per litre test solution.


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water, quality according ISO 7346/1. The water was saturated with oxygen. pH-value: 8.1 - 8.2.
pH , temperature and oxygen saturation were observed at the beginning, after 2-4, 24, 48, 72 and 96 h in all vessels


OTHER TEST CONDITIONS
- Photoperiod (hrs dark / hrs light): 12/12
- Light intensity: 700 Lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and behavior of the test fish and control fish were observed at 2-4, 24, 48, 72, 96 h.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- There was no mortality in control as well as in treated fish at any observation time point.
- Behavioural abnormalities: tail-heavy swimming posture, hypoactivity

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In this study the test item did not cause mortality to zebrafish after 96 h when tested with a 500 mg/L (nominal) solution

Executive summary:

The acute toxicity of the test item to fish (zebrafish) was determined according to OECD-Guideline for Testing of Chemicals No. 203 (1984) at Hoechst AG, Frankfurt, Germany. A static limit test with a 500 mg/L (nominal) solution was conducted. The preparation method has been chosen with regard to the test item properties and in accordance with the relevant OECD guidance document. Duration of the test was 96 h. 10 test organisms were exposed to the test solution and the control respectively. Water quality parameters pH-value, temperature and oxygen-saturation measured after 0, 2 -4, 24, 48, 72 and 96 h were determined to be within the acceptable limits.

The 500 mg/L solution of the test item didn´t show lethality at all observation time points up to 96 hours.