Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
27 August 2015 - 9 October 2015
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: solid
Details on test material:
Chemical name (IUPAC) (Z)-3-(3-Chloro-2-fluoro-phenyl)-2-(4-chloro-2-fluoro-phenyl)- acrylonitrile
Lot no. FIS201405080058
Molecular formula C15H7Cl2F2N
Molecular mass 310.13 g/mol
Water solubility <0.02 mg/l at 20°C and pH 4.4–6.0, as determined in the course of an OECD 105 study (ACH study no. A15-01329).
Purity 101.2 % (m/m)
Theoretical oxygen demand 2.064 mg O2/mg (calculated according to chapter 5.2 assuming nitrification)
Test item storage at room temperature, protected from light and humidity
Stability Stable under storage conditions
Expiry date 11 June 2016

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
50 mg/L
Based on:
ThOD/L

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
ca. 20
Sampling time:
10 d

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other: not readily biodegradable
Executive summary:

The biodegradability of PAC-Ethenenitrile (CAS no. 1219086-87-9) exposed to microorganisms derived from activated sludge of a municipal sewage treatment plant was investigated under aerobic static exposure conditions.

The biodegradability of PAC-Ethenenitrile based on O2 consumption was calculated to be 22% after 28 days as compared to the theoretical oxygen demand (ThOD).

The biodegradation of PAC-Ethenenitrile reached 20% at the end of the 10-d window. Biodegradation of the test item was observed without any lag-phase.The procedure control sodium benzoate reached 83% biodegradation after 14 days, thus confirming

suitability of inoculum and test conditions.

All validity criteria were fulfilled.

PAC-Ethenenitrile did not reach the pass level of 60% for ready biodegradability in the Manometric Respirometry Test neither within the 10-d window nor after 28 days of incubation, and, therefore, cannot be termed as readily biodegradable.