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Acute Fish Toxicity:

Water accommodated fractions of hydrocarbons, C9-C11, n-alkanes, isoalkanes, cyclics, <2% aromatics, produced a 96-hour LL50 value of >1000 mg/L and LL0 value of 100 mg/L with rainbow trout, Oncorhynchus mykiss. No mortality was observed in the highest dose tested. These results are read-across to Hydrocarbons, C8-C11, n-alkanes, isoalkanes, <2% aromatics.

Chronic Fish Toxicity:

The hydrocarbons, C9 -C11, n-alkanes, isoalkanes, cyclics, <2% aromatics, estimated freshwater fish 28-day NOELR (No Observed Effect Loading Rate) value is 0.131 mg/l based on growth. These results are read-across to Hydrocarbons, C8-C11, n-alkanes, isoalkanes, <2% aromatics.

Acute Invertebrate Toxicity:

The water accommodated fraction (WAF) of Hydrocarbons, C8-C11, n-alkanes, isoalkanes, <2% aromatics, did not produce a 50% effect (immobility) with Daphnia magna at a loading of 100 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >100 mg/L.

Data are also available for the invertebrate toxicity of the surrogate substance, hydrocarbons, C9-C11, n-alkanes, isoalkanes, cyclics, <2% aromatics. The water accommodated fraction (WAF) of hydrocarbons, C9-C11, n-alkanes, isoalkanes, cyclics, <2% aromatics, did not produce a 50% effect (immobility) with Daphnia magna at a loading of 1000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >1000 mg/L. There was no mortality at the 1000 mg/L loading level after 48 hours. Therefore, the 48-hour LL0 for mortality is reported as 1000 mg/L. There was also no mortality in the control.

Chronic Invertebrate Toxicity:

The hydrocarbons, C9-C11, n-alkanes, isoalkanes, cyclics, <2% aromatics, estimated freshwater invertebrate 21-day NOELR (No Observed Effect Loading Rate) value is 0.230 mg/l based on reproduction. These results are read-across to Hydrocarbons, C8-C11, n-alkanes, isoalkanes, <2% aromatics.

Algal Toxicity:

Growth of algal cultures, as measured by growth rate, exposed to water accommodated fractions of Hydrocarbons, C8-C11, n-alkanes, isoalkanes, <2% aromatics, was not inhibited by more than 50% over 72 hours. Therefore, the 72-hr EL50 values for this endpoint is reported as >100 mg/L. The 72-hr NOELR value for growth rate is 100 mg/L.

Data are also available for the algal toxicity of the surrogate substance, hydrocarbons, C9-C11, n-alkanes, isoalkanes, cyclics, <2% aromatics. Growth of algal cultures, as measured by biomass and growth rate, exposed to water accommodated fractions of hydrocarbons, C9-C11, n-alkanes, isoalkanes, cyclics, <2% aromatics, was not inhibited by more than 50% over 72 hours. Therefore, the 72-hr EL50 values for these two endpoints are reported as >1000 mg/L. The 72-hr NOELR values for biomass and growth rate are 3 and 100 mg/L, respectively.

Microorganisms Toxicity:

The hydrocarbons, C9-C11, n-alkanes, isoalkanes, cyclics, <2% aromatics, estimated protozoan, Tetrahymena pyriformis, 48-hr EL50 value for this substance is 0.950 mg/L based on growth inhibition. These results are read-across to Hydrocarbons, C8-C11, n-alkanes, isoalkanes, <2% aromatics.