1-Propanamine, 3-(ethenyloxy)-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3-(ethenyloxy)propan-1-amine

EC Number:

633-172-6

Cas Number:

66415-55-2

Molecular formula:

C5 H11 N O

IUPAC Name:

3-(ethenyloxy)propan-1-amine

Details on test material:

- Identification: Aminopropyl Vinyl Ether
- Chemical name: 3-(ethenyloxy)propan-1-amine
- Physical form: liquid
- Appearance: colourless, clear
- Water solubility: miscible
- Density: 0.891 g/cm3 (20°C)
- Purity: 99.6 corrected area-%
- Homogeneity: homogeneous
- Storage conditions: The test substance was stored at ambient temperature (avoid temperature 25°C)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

At the start of the exposure (0 h) samples from vessels without daphnids and at the end of the exposure (48 h) samples from vessels with daphnids were analyzed. The samples were transported to the Analytical Laboratory on the day of sampling.

Test solutions

Details on test solutions:

The volume of the test substance required for preparation of the nominal concentration was calculated from the density (d=0.891 g/cm3). Prior to use, the test substance container was shaken to ensure homogeneity.The stock solution (100 mg/L) was prepared by directly pipetting 224µL of test substance to 2L of test medium and stirring for approximately 10 min (magnetic stir plate) until visibly dissolved. The lower test concentrations were prepared by dilution of this stock solution. Before the dilution is performed, the stock solution was checked for complete dissolution of the test substance. The stock solution appeared colourless-clear.
- Test groups: 0 (control), 4.6, 10, 22, 46 and 100 mg/L as nominal concentrations based on test substance mass without a correction for purity.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.20-3.20 mmol/L

Test temperature:

19.6-20.1°C

pH:

7.5-8.5

Dissolved oxygen:

8.6-8.8 mg/L

Details on test conditions:

- Replicates: 4 replicates with test animals (5 animals per replicate)
- Test vessels: glass beakers (nominal volume 100mL)
- Test volume: 50 mL
- Biological loading: 5 animals/test vessel (0.1 animals/mL)
- Aeration: none
- Diet: No feeding during the exposure time.

Reference substance (positive control):

yes

Remarks:

Sodium chloride

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes