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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro DNA damage and/or repair study
Remarks:
Type of genotoxicity: DNA damage and/or repair
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
2002
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study in Chinese (brief english summary incl. different tables)

Data source

Reference
Reference Type:
publication
Title:
Study on three kinds of gasoline oxygenates induced DNA damage in mice fibroblasts
Author:
Song C, Zhang Z, Chen X, Zhang Y, Wang C & Keming L
Year:
2002
Bibliographic source:
China J Ind Hyg Occup Dis 20, 362-364

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Comet assay for detection of DNA damage in L 929 mice fibroblasts
GLP compliance:
not specified
Type of assay:
comet assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
not further specified

Method

Species / strain
Species / strain / cell type:
mammalian cell line, other: L 929 mice fibroblasts
Metabolic activation:
without
Test concentrations with justification for top dose:
up to 150 mg/mL
Vehicle / solvent:
DMSO
Controls
Negative solvent / vehicle controls:
yes
Details on test system and experimental conditions:
not further specified
Evaluation criteria:
not further specified
Statistics:
yes

Results and discussion

Test results
Species / strain:
mammalian cell line, other: L 929 mice fibroblasts
Metabolic activation:
without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
valid
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

In a Comet assay with L 929 mice fibroblasts, dimethyl carbonate gave no indications for a DNA damage at concentrations of up to 150 mg/mL
Executive summary:

In a Comet assay with L 929 mice fibroblasts, dimethyl carbonate gave no indications for a DNA damage at concentrations of up to 150 mg/mL