Poly(oxy-1,2-ethanediyl), .alpha.-[3-[(carboxymethyl)methylamino]-2-hydroxypropyl]-.omega.-(4-nonylphenoxy)-, sodium salt (1:1)

Short-term toxicity to aquatic invertebrates

Description of key information

The 48h-EC50 of 3.92 mg/L was determined for Daphnia magna in the study according to OECD 202 (GLP, reliability 1). This value is based on active ingredient content.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

3.92 mg/L

Additional information

Two studies are available on the acute toxicity to Daphnia magna. The key study (reliability 1) is performed with the registered substance and the 48h-EC50 is reported as 3.92 mg ai/L. In the second study (reliability 2) the 48h-EC50 was determined for a structurally similar alkyl sarcosine derivative. The 48h-EC50 was determined to be 1.8 mg/L (test material). The read-across is based on the structural similar 2-hydroxy propyl-N-methyl glycinate moiety present in both substances. The substances are different in the alkyloxy moiety attached to the propanol structure, but the 48h-EC50 for Daphnia show that the toxicity is in the same range for both substances.

In the key study, the effects of the test item Nonylphenol ethoxylate, sarcosine derivative were determined according to OECD 202 under GLP conditions. The study was assigned reliability 1 as the validity criteria of the test guideline were fulfilled. The water quality parameters (pH-value and dissolved oxygen concentration), measured at 0 and 48 h, were within the acceptable limits. Furthermore, complete identification of the test substance and chemical analysis of exposure concentrations were included (Noack, M. 2013).

The study was conducted under static conditions over a period of 48 h with 5 concentration levels of the test item nonylphenol ethoxylate, sarcosine derivative in the range of 1.25 to 20.0 mg/L, prepared with dilution water in a geometric series with a factor of 2. Twenty daphnids were exposed to each concentration level, the pH-neutralized concentration and the control.

 

The concentrations of the test item nonylphenol ethoxylate, sarcosine derivative were analytically verified by LC-MS/MS in the fresh media (0 h) and in the old media (48 h) of all concentration levels and the control.

The measured concentrations of the test item nonylphenol ethoxylate, sarcosine derivative in the fresh media (0 h) were in the range of 102 to 116 % of the nominal values. The recoveries of the test item in the old media (48 h) were in the range of 78 to 102 % of the nominal values. The geometric mean measured concentrations were calculated to be: 1.20 – 2.38 – 4.92 – 9.77 – 20.4 mg/L, which corresponds to mean recovery rates in the range of 95 to 102 %.

 

The endpoints were assessed based on the geometric mean measured concentrations of the test item nonylphenol ethoxylate, sarcosine derivative. The 48h-EC50 was reported to be 4.19 mg/L (95% CL 2.70 - 4.81) derived from the geometric mean measured concentrations of the test material. This corresponds to 48h-EC50 of 3.92 (95% CL 2.53 - 4.50) based on the active ingredient concentration.