Phenolates from Hydrocarbon Resin production

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

publication

Title:

Cooperative assessment of herbicide phytotoxicity to Selenastrum capricornutum using microplate and flask bioassay procedure

Author:

St. Laurent, D.; Blaise, C.; Macquarrie, P.; Scroggins, R.; Trottiers, B.

Year:

1992

Bibliographic source:

Environ. Toxicol. Water Qual., 7, 35-48

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: US EPA (1989), Algal growth test, p. 147-174. EPA/600/4-89/001, modified

Deviations:

yes

Remarks:

initial cell density 20 000 cells/ml instead of 10 000 cells/ml

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Phenol

EC Number:

203-632-7

EC Name:

Phenol

Cas Number:

108-95-2

Molecular formula:

C6H6O

IUPAC Name:

phenol

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Common name: Pseudokirchneriella subcapitata (reported as Selenastrum capricornutum)
- Strain: ATCC 22662

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Details on test conditions:

- Test vessel: Erlenmeyer flasks
- Type: loosely closed
- Material, size, headspace, fill volume: glass, 125 ml flasks filled with 40 mL culture
- Aeration: no
- Initial cells density: 20 000 cells/ml
- Control end cells density: no data
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6

GROWTH MEDIUM
- Standard medium used: yes (0.625X AAP medium)
Macronutrient (acc. to US EPA guideline).

OTHER TEST CONDITIONS
- Photoperiod: continuous
- Light intensity and quality: cool white fluorescene lamps, 73 +/- 9 µE/mxs
- Flasks were continuousla shaken 100 rpm

EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: Coulter Counter

- Test concentrations: 10 concentrations (no details)

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

61.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number