Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 607-089-0
CAS number: 224789-21-3
Table¿1¿1: Inhibition of the growth rate [%] of Desmodesmus subspicatus after 72 hours exposure to Imidazotriazinon
of Imidazotriazinon (mg/L) *
in the test vessels
* Calculated from TOC values
The purpose of this study was to determine the effects of the test
compound Imidazotriazinon on the growth of the green algae Desmodesmus
subspicatus. The study was conducted in agreement with the test
guideline OECD no. 201.
The test substance Imidazotriazinon was incubated in an aqueous
solution including nutrients with an algae population of Desmodesmus
subspicatus for a test duration of approximately 72 hours in an
incubator shaker apparatus.
The nutrient solution was made up of mainly ammonium, phosphates
and some trace elements.
For the preparation of the test solutions a suspension with a
nominal loading of 100 mg/L Imidazotriazinon was suspended in
demineralized water, sonicated for approximately 30 min and constantly
stirred for approximately 24 hours. This suspension was filtered through
a glass-fiber filter. The filtered solution was used for the preparation
of the test solutions. The highest concentration was a 1:1.25 dilution
obtained by adding nutrient solution and inoculum, the further
concentrations were obtained by dilutions of 1:5, 1:10, 1:25, 1:50 and
1:100 with demineralized water, nutrient solution and inoculum.
Additionally, a control solution was prepared with demineralized water
without test material.
The organic carbon concentration of the ultrasonified and filtered
stock solution was analyzed with a TOC analyzer. The final
concentrations in the test vessels were calculated based on the dilution
factor. The algae were exposed to each concentration in triplicate. Six
vessels were prepared for the control. The algae were incubated under
standardized conditions (continuous light, controlled temperature) in an
incubator shaker apparatus. As a parameter for the growth of the algae
population, the algae cells were measured with a coulter counter. The
growth rate was calculated on the basis of the cell counts.
The calculated growth rate of each concentration was compared to
those of the controls, and the inhibition was calculated.
Imidazotriazinon had a concentration-depended inhibitory effect on
the growth of Desmodesmus subspicatus. The ErC50(growth rate) was 11.354
mg/L [95 % confidence limits: 8.838-16.176] on the basis of measured
concentrations. The NOEC was 0.93 mg/L, the LOEC was 1.86 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again