Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

Using the EU Method C.2 (Acute Toxicity for Daphnia) a 72h-EC0 of 2.8 mg/L (measured) was determined. No toxic effect and no abnormal behaviour to Daphnia magna was observed (up to the water solubility under exposure conditions).

Key value for chemical safety assessment

Additional information

In order to test acute toxicity to invertebrates of the substance, Daphnia magna was exposed to the test solution of 1 nominal concentration of the test substance (2 mg/L) and blank control solution for a period of 48 h under static conditions. The method used was the EU Method C.2 (Acute Toxicity for Daphnia). Mobility and visible abnormalities were recorded at 24 and 48 h. The measured concentrations at 48 hours was 2.8 mg/L. No toxic effect and no abnormal behaviour was observed (up to the water solubility under exposure conditions). This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute Daphnia study.

The substance has been tested up to the water solubility under exposure conditions. In rage finding tests the maximum of water solubility of the substance under exposure conditions was determined to be about 2 mg/L. To achieve this concentration, the five fold amount of the substance (10mg/L) was weighed in. In the report of this study a water solubility of the substance of 7 mg/L was presented. This value has been determined within a GLP study. The medium in the respective study differs from the test medium used in the presented study concerning the daphnia toxicity.

The results of the main test as well as those of the range finding test clearly demonstrated that under exposure conditions the maximum water solubility is lower. If a substance is tested at the maximum water solubility, under exposure conditions slight fluctuations of the respective concentrations are to be expected. The observed difference between the nominal concentration of 2 mg/L and the mean measured concentration of 2.8 mg/L can be explained by this and is seen as negligible.