Registration Dossier
Registration Dossier
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EC number: 230-847-3 | CAS number: 7336-20-1
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- GLP compliance:
- no
Test material
- Reference substance name:
- Disodium 4,4'-diaminostilbene-2,2'-disulphonate
- EC Number:
- 230-847-3
- EC Name:
- Disodium 4,4'-diaminostilbene-2,2'-disulphonate
- Cas Number:
- 7336-20-1
- Molecular formula:
- C14H14N2O6S2.2Na
- IUPAC Name:
- disodium 2,2'-ethene-1,2-diylbis(5-aminobenzenesulfonate)
- Details on test material:
- - Name of test material: 4,4-diamino-stilben-2,2-disulfonic acid, disodium salt
- Analytical purity: no data
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, industrial, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge from BASF sewage works
- Concentration of sludge: 66,7 mg/L in the test - Duration of test (contact time):
- 53 d
Initial test substance concentration
- Initial conc.:
- 400 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Solubilising agent: none used
- Test temperature: room temperature
- pH: 6.7 - 7.8
- pH adjusted: yes
TEST SYSTEM
- Culturing apparatus: all glass bottles (volume: 5L)
- Number of culture flasks/concentration: 1
- Measuring equipment: no data available
- Test performed in open system: no
SAMPLING
- Sampling frequency: after 3hours, 1, 4, 8, 15, 18, 27, 31, 36, 46 and 53 days
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
Results and discussion
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- -1
- Sampling time:
- 53 d
Any other information on results incl. tables
| 0 h | 3 h | 1 d | 4 d | 8 d | 15 d | 18 d | 27 d | 31 d | 36 d | 46 d | 53 d | |
| DOC measured [mg/l] | 423 | 405 | 408 | 384 | 415 | 359 | 349 | 327 | 350 | 347 | 346 | 359 |
| TOC elimination [%] | -6 | 1 | -1 | -1 | -7 | 7 | 8 | 12 | 5 | 4 | 2 | -1 |
| pH | 6.7 | 7.2 | 7.8 | 7.8 | 4.8 | 6.2 | 5.4 | 5.8 | 6.6 | 6.6 |
Applicant's summary and conclusion
- Interpretation of results:
- under test conditions no biodegradation observed
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