3-chloroaniline

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions.

Data source

Reference

Reference Type:

publication

Title:

#44: Long-term toxicity to aquatic invertebrates (Daphnia magna)

Author:

MITI

Year:

2000

Bibliographic source:

http://www.safe.nite.go.jp/jcheck/

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3-chloroaniline

EC Number:

203-581-0

EC Name:

3-chloroaniline

Cas Number:

108-42-9

Molecular formula:

C6H6ClN

IUPAC Name:

3-chloroaniline

Test material form:

other: liquid

Details on test material:

- Purity not reported
- Empirical formula: C6H6ClN
- Molecular mass: 127.58 g/mole

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

21 d

Test conditions

Test temperature:

20 ± 1 °C

Nominal and measured concentrations:

Concentrations: 0.0032, 0.010, 0.032, 0.10, 0.32 and 1.0 mg/L

Details on test conditions:

- Test volume: 80 mL per container
- Stations: 10 container per test concentration
- Number of animals: 10 animals per test concentration (1 animal per container)
- Temperature: 20 ± 1 °C
- Photoperiod: 16 h light / 8 h dark
- Test typ: semi-static, water change every 3 days

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

0.16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: (0.10 ~ 0.25 mg/L)

Effect concentrations 2

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

0.012 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: (0.007 ~ 0.019 mg/L)

Effect concentrations 3

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.003 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 4

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

0.01 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

The measured concentrations confirmed that deviation from the nominal concentrations was less than 20 %.

Conclusions:

The long-term toxicity of aquatic intvertebrates (Daphnia magna) of 3-chloroaniline was determined under semi-static conditions. After 21 days exposure period a LC50 of 0.16 mg/L and an EC50 og 0.12 mg/L were measured, and a NOEC of 0.0032 mg/L and a LOEC of 0.010 mg/L was calculated.

Executive summary:

The long-term toxicity of aquatic intvertebrates (Daphnia magna) of 3-chloroaniline was determined under semi-static conditions. After 21 days exposure period a LC50 of 0.16 mg/L and an EC50 og 0.12 mg/L were measured, and a NOEC of 0.0032 mg/L and a LOEC of 0.010 mg/L was calculated.