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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: non guideline, no GLP

Data source

Reference
Reference Type:
publication
Title:
The Metabolism of Cyclo-octene, 1, 3-cyclo-octadiene and 1, 5-cyclo-octadiene in the Rat and Rabbit, and the Reaction of these Compounds with Glutathione in vivo in the Rat
Author:
Waring, R.H.
Year:
1971
Bibliographic source:
Xenobiotica 1, 303-308

Materials and methods

GLP compliance:
no

Test material

Constituent 1
Reference substance name:
Cyclooctene
EC Number:
213-245-5
EC Name:
Cyclooctene
Cas Number:
931-88-4
IUPAC Name:
cyclooctene
Details on test material:
- Name of test material (as cited in study report): cyclooctene

Test animals

Species:
other: rats and rabbits
Strain:
not specified
Sex:
female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
not specified
Duration and frequency of treatment / exposure:
single dose
Doses / concentrations
Remarks:
Doses / Concentrations:
2.0 mmol/kg bw
No. of animals per sex per dose / concentration:
6
Control animals:
no

Results and discussion

Metabolite characterisation studies

Metabolites identified:
yes
Details on metabolites:
Two sulfur containing metabolites were detected in urine of both female rats and rabbits following a single dose of cyclooctene.
These were identified as 2-hydroxycyclo-octyl mercapturic acid and 3-hydoxycyclo-octyl mercapturic acid by co-chromatography with synthetic standards and mass spectrometry.
Cis/trans isomers were present in 1:1 ratio.

Any other information on results incl. tables

Sulfur containing metabolites in urine (24h)

 

Dose (mmol/kg bw)

Female Rabbit (n=6)

Female Rat (n=6)

Glucuronide (% of dose)

Ethereal sulphate
(% of dose)

Mercapturic acids
(% of dose)

Mercapturic acids
(% of dose)

2-Hydroxy-

3-Hydroxy-

2-Hydroxy-

3-Hydroxy-

Cyclooctene

2.0

51.2

20.3

2.3

8.3

4.9

7.2

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): no bioaccumulation potential based on study results
More than 80% of an oral dose of 2 mmol cyclooctene (220mg)/kg bw was excreted within 24h as sulfur containing metabolites indicating extensive absorption and extensive metabolism including hydroxylation as well as rapid excretion.
Executive summary:

In a published study the formation of sulfur containing metabolites in female rabbits and rats was investigated.

Animals received a single dose of 2 mmol (220mg) per kg bw, 24h urine was collected and analysed. More than 80% of the dose was recovered as sulfur containing metabolites of which 2-hydroxy and 3-hydroxy-mercapturic acids were identified.