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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Short description of an in-vivo study performed similar to the OECD guideline. However several information details are missing in this summary.
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1974
Report date:
1974

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Deviations:
yes
Remarks:
as only a summary report is available several information details were not given, e.g. controls, detailed application description, information on washing, individual results.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium hydrogensulphide
EC Number:
240-778-0
EC Name:
Sodium hydrogensulphide
Cas Number:
16721-80-5
Molecular formula:
HNaS
IUPAC Name:
sodium hydrogensulphide
Constituent 2
Reference substance name:
sodium hydrogensulfide
IUPAC Name:
sodium hydrogensulfide
Constituent 3
Reference substance name:
sodium sulfhydrate
IUPAC Name:
sodium sulfhydrate
Details on test material:
- Name of test material (as cited in study report): Sulfhydrat F150, Natriumsulfhydratlauge
- Molecular formula (if other than submission substance): NaHS (aqueous solution)
- Molecular weight (if other than submission substance): 56.06 g/mol
- Substance type: technical product
- Physical state: yellow liquid
- Storage condition of test material: In tightly closed original container, in a cool, well-ventilated place; protect from sunlight; do not store with acids; do not store together with oxidizing agents
- Other: pH value: 12 - 13

Test animals / tissue source

Species:
rabbit
Strain:
Himalayan
Details on test animals or tissues and environmental conditions:
TEST ANIMALS-albino
- Housing: in single cages
- Diet: mixed diet ERKA 8300 (Futtermittelwerke Robert Koch oHG/Hamm, Germany), ad libitum
- Water: tap water, ad libitum

Test system

Vehicle:
physiological saline
Controls:
not specified
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 mL
- Concentration (if solution): undiluted Sulfhydrat solution (ca. 31-32% NaHS), 10 % Sulfhydrat solution, 5 % Sulfhydrat solution, and 1 % Sulfhydrat solution
Duration of treatment / exposure:
single treatment
Observation period (in vivo):
144 hours
Number of animals or in vitro replicates:
6
Details on study design:
- the test substance was placed in the conjunctival sac
- eyes were examined at 1, 3, 7, 24, 48, 72 and 144 hours after test substance application
-for the 24 hours examination, the eyes were further examined with the aid of fluorescein

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
other: no data
Remarks on result:
other: After 6 days the observation was stopped. The effect of the undiluted Sulfhydrat solution (31-32 % NaHS) was not reversible at this time. Even if the observation period should be up to 21 days this result is interpreted as a corrosive effect.
Remarks on result:
other: Short description of an in-vivo study performed similar to the OECD guideline. However several information details are missing in this summary.
Irritant / corrosive response data:
- the undiluted sample resulted in general to deep red colouring and an obvious lacteal swelling of the conjunctivae for 144 hours; furthermore a mild to severe opaque cornea was observed
- the 10 % solution resulted in a mild general red colouring of the conjunctivae for a little bit longer than 24 hours; in addition two rabbits showed a very mild redness of the conjuntivae for about three hours
- the 5 % solution resulted in a very mild redness of conjunctivae in 3 rabbits for approximately 3 hours
- the 1 % solution resulted in no irritating or corrosive effects

Any other information on results incl. tables

The authors considered the undiluted product as strongly irritant and the 5% and 10 % solutions as very slight to mildly irritant to eyes.

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye)
Remarks:
Migrated information Criteria used for interpretation of results: expert judgment
Conclusions:
After 6 days the observation was stopped. The effect of the undiluted Sulfhydrat solution (31-32 % NaHS) was not reversible at this time. Even if the observation period should be up to 21 days this result is interpreted as a corrosive effect. The 1 % solution can be judged as not corrosive and not irritant.