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Ecotoxicological information

Long-term toxicity to fish

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Administrative data

Endpoint:
fish short-term toxicity test on embryo and sac-fry stages
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Confounding effects of hypoxia. Control survival in tests under normoxic conditions was too low in most tests. Highly variable stocking density. No replicates in most tests.

Data source

Reference
Reference Type:
publication
Title:
Effect of hydrogen sulfide on northern pike eggs and sac fry.
Author:
Adelman IR, Smith LL Jr
Year:
1970
Bibliographic source:
Transactions of the American Fisheries Society 99: 501-509

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 212 (Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages)
Principles of method if other than guideline:
In this study, northern pike eggs of 24 up to 144 h after fertilization were exposed to a control and a series of sulfide concentrations for 96 h up to 17 days under either hypoxic or normoxic conditions.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
H2S

Test organisms

Test organisms (species):
Esox lucius
Details on test organisms:
Northern pike eggs

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
17 d
Remarks on exposure duration:
17 days = maximum test duration

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.022 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Remarks:
H2S
Basis for effect:
mortality
Remarks on result:
other: Lowest LC50, 96-h test, eggs of 60 h after fertilization, hypoxia
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.058 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Remarks:
H2S
Basis for effect:
mortality
Remarks on result:
other: Highest LC50, 96-h test, eggs of 24 h after fertilization, hypoxia

Applicant's summary and conclusion

Conclusions:
In this study, northern pike eggs (Esox lucius) of 24 up to 144 h after fertilization were exposed to a control and a series of sulfide concentrations for 96 h up to 17 days under either hypoxic or normoxic conditions. The LC50 values varied from 0.022 (96 h exposure, eggs of 60 h after fertilization, hypoxia) to 0.058 mg H2S/L (96 h exposure, eggs of 24 h after fertilization, hypoxia). All LC50 values (whether obtained under normoxic or hypoxic conditions) were very similar.