Registration Dossier
Registration Dossier
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EC number: 259-487-5 | CAS number: 55117-15-2
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 1996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Alpha,2-dichloro-6-fluorotoluene
- EC Number:
- 259-487-5
- EC Name:
- Alpha,2-dichloro-6-fluorotoluene
- Cas Number:
- 55117-15-2
- Molecular formula:
- C7H5Cl2F
- IUPAC Name:
- 1-chloro-2-(chloromethyl)-3-fluorobenzene
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 98
- Species / strain / cell type:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 Mix
- Test concentrations with justification for top dose:
- 3.3, 10.0, 33.3, 100.0, 333.3, 1000.0, 2500.0 and 5000.0 ug/plate
- Details on test system and experimental conditions:
- Each concentration and the controls were tested in triplicate.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- from 1000 to 5000 ug/plate
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 5000 ug/plate
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- from 1000 to 5000 ug/plate
Any other information on results incl. tables
At the higher concentration the background growth was reduced with and without S9 mix in all strain used.
No substantial increase in revertant colony numbers of any of the two tester strains was observed following treatment with the substance at any concentration level, neither with or without S9 mix.
There was also no tendency of higher mutation rates with increasing concentrations in the range below the generally acknowledged border of biological relevance.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
In can be stated that the test article did not induce gene mutations by base pair changes or frameshifts in the genome of the strains used.
2-chloro-6-fluoro benzylchloride is considered to be non-mutagenic in this Salmonella typhimurium reverse mutation assay.
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