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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999-12-07 to 1999-12-09
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, GLP, no analytical dose verification

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Adipic acid, Cyclicester with diethylenglycol
- Analytical purity: > 90%
- Impurities (identity and concentrations): Diethylenglycol <10% ; water<2%
- Purity test date: Jan 1999
- Lot/batch No.: B47

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2 - 24 h
- Age of stock animals: 2-4 weeks

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.38 mmol/l
Test temperature:
20.5 - 20.6°C
pH:
8.0 - 8.2 at test start; 8.1 - 8.2 at test end
Dissolved oxygen:
8.6 - 8.7 at test start; 8.3 - 8.4 at test end
Nominal and measured concentrations:
0, 12.5, 25, 50, 100 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Flat bottom glass tubes, nominal volume 20 ml
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium
- Ca/mg ratio: 4:1
- Conductivity: 600 µSiemens/cm

OTHER TEST CONDITIONS
- Photoperiod: 16:8
- Light intensity: About 2 - 7 µE/(m2*s) at a wave length of 400 - 700 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Swimming ability after 0, 24 and 48 h
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

None of the daphnids were immobilized by the treatment, even at the highest concentration tested.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
• In the control the immobilization was < 10% . • In the control no daphnids were captured in a surface film. • The 02-concentration was > 3 mg/I in all tested replicates . • The pH-value in the test solutions should not vary within more than one unit .