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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

ErC50 27.77 mg/L, NOEC 6.25 mg/L, OECD 201, EU Method C. 3, EPA OPPTS 850. 5400, Sipos 2013c.

Key value for chemical safety assessment

EC50 for freshwater algae:
27.77 mg/L
EC10 or NOEC for freshwater algae:
6.25 mg/L

Additional information

In the key study (Sipos, 2013c) the toxicity of the test material to algae was determined in a growth inhibition study performed with Pseudokirchneriella subcapitata under GLP conditions and in accordance with the standardised guidelines OECD 201, EU Method C.3 and EPA OPPTS 850. 5400.

Algal cultures were exposed to the test material at concentrations ranging from 6.25 to 100 mg/L for 72 hours under static freshwater conditions. Cell numbers were manually counted at 24, 48 and 72 hours. Microscopic observations of algal cells were also performed at 24, 48 and 72 hours to detect any abnormal appearance of the algae.

Analytical monitoring was conducted by HPLC and MS analysis at all concentration levels. Samples were collected for analysis at study initiation, 24 hour intervals, and then again at termination. Control samples were analysed at initiation and termination only. Results showed that the test material absorbed to the increasing algal biomass. Therefore effect concentrations have been reported in terms of nominal concentrations.

With respect to the inhibitory effect of the test material, the 0-72 h average specific growth rates, areas and yield were significantly different from that of the control group in the examined concentration range of 20.4 - 100.0 mg/L (nominal). Based on the growth rate of the algae the following effect parameters were determined, ErC50 27.77 mg/L, NOEC 6.25 mg/L, and LOEC 12.5 mg/L.